Determination of melamine and its conjugates in calcium oxalate stones by Fourier transform infrared spectroscopy
10.19428/j.cnki.sjpm.2022.21529
- VernacularTitle:傅里叶导数红外光谱法测定草酸钙结石中三聚氰胺及其结合物
- Author:
Xinyu WANG
1
;
Qilai LONG
2
;
Luning WANG
1
;
Liujin MA
1
;
Jiani XU
1
;
Ting LIU
1
;
Yifei WANG
3
;
Feifei HUANG
3
;
Jianming GUO
2
;
Bo CHEN
3
;
Liang CHEN
1
Author Information
1. College of Biological and Environmental Sciences， Ningbo， Zhejiang 315100，China
2. Department of Urology， Zhongshan Hospital， Fudan University， Shanghai 200032， China
3. Key Laboratory of the Ministry of Public Health and Safety Education， School of Public Health， Fudan University， Shanghai 200032，China
- Publication Type:Journal Article
- Keywords:
calcium oxalate stone;
melamine;
cyanic acid;
uric acid;
Fourier transform infrared spectroscopy;
two-times second derivative
- From:
Shanghai Journal of Preventive Medicine
2022;34(8):820-824
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo establish a method of two-times second derivative Fourier transform infrared spectroscopy （FTIR） for identifying melamine （MEL） and its conjugates with cyanuric acid （MEL-CYA） or with uric acid （MEL-UA） in human urinary calculi. MethodsMEL， MEL-CYA and MEL-UA were added to calcium oxalate stone samples， and then analyzed by two-times second derivative FTIR for identifying the characteristic response bands of MEL and its conjugates in stones as well as confirming the ratio of detection. ResultsThe second derivative FTIR could improve the detection of MEL in CaOx stones by two orders of magnitude. When CaOx being mixed with MEL （MEL∶ CaOx）， the two-times second derivative FTIR showed the characteristic peak at 1 548 cm-1 and the minimum mass ratio of detection at 1∶100 （1%）. When CaOx being mixed with MEL-CYA complex （MEL-CYA∶ CaOx）， the two-times second derivative FTIR showed the characteristic peak on 1 740 cm-1 and 1 538 cm-1 and the minimum mass ratio of detection at 1∶500 （0.2%）. When CaOx being mixed with MEL-UA complex （MEL-UA∶ CaOx）， the two-times second derivative FTIR showed the characteristic peak at 1 117， 982 and 710 cm-1 and the minimum mass ratio of detection at 1∶250 （0.4%）.ConclusionCompare to the original spectra， the two-times second derivative FTIR can improve the detection ratio MEL in the CaOx stones from 0.2% to 1.0%. The second derivative FTIR has the unique characteristic bands and sensitive detection limit for identifying MEL in kidney stones.
