Identification of chemical constituents and blood-absorbed ingredients of FeiPi QiXu decoction by UHPLC-Q-TOF/MS
10.12206/j.issn.2097-2024.202204079
- VernacularTitle:基于UHPLC-Q-TOF/MS技术鉴定肺脾气虚汤的化学成分及其入血成分
- Author:
Jin WU
1
;
Jing ZHAO
2
;
Tao PANG
2
Author Information
1. Fujian drug Inspection Center, Fuzhou 350003, China.
2. .
- Keywords:
FeiPi QiXu decoction;
UHPLC-Q-TOF/MS;
chemical constituents;
blood-absorbed ingredients
- From:
Journal of Pharmaceutical Practice and Service
2022;40(5):446-453
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the chemical constituents and blood-absorbed ingredients of FeiPi QiXu decoction, and provide reference for clarifying the material basis of its efficacy. Methods An ultrahigh performance liquid chromatography-quadrupole-time of flight-mass spectrometry (UHPLC-Q-TOF/MS) method was applied to analyze the data of FeiPi QiXu decoction extraction and the serum samples of SD rats after oral administration. The chemical constituents and blood-absorbed ingredients were quickly identified by chemical reference substance, self-built mass spectrometry database, MSFINDER database and the relevant literature. Results A total of 56 chemical constituents were identified from Feiqi Deficiency Decoction, mainly were flavonoids, triterpenoids and phenols. Most of the compounds came from Radix Astragali praeparata cum melle, Citri Reticulatae Pericarpium, Poria and Codonopsis Radix. 11 prototype compounds were identified in the serum, including Formononetin, Astragaloside IV and Neohesperidin. Conclusion This study comprehensively clarified the chemical composition and source of medicinal materials, and preliminarily analyzed its blood-absorbed ingredients, which could provide a scientific basis for further study on the pharmacodynamic material basis and quality control of FeiPi QiXu decoction.
