Preparation and characterization of photosensitive ROS-responsive rapamycin liposomes
10.12206/j.issn.2097-2024.202110051
- VernacularTitle:光敏ROS响应型雷帕霉素脂质体的制备及表征
- Author:
Xiqing GAO
1
;
Guangzhao LU
1
;
Ying LU
1
;
Hao ZOU
1
Author Information
1. Department of Pharmaceutics, School of Pharmacy, Naval Medical University, Shanghai 200433, China.
- Keywords:
liposome;
rapamycin;
near infrared light;
ROS;
release in vitro
- From:
Journal of Pharmaceutical Practice and Service
2022;40(5):437-441
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the preparation process and properties of photosensitive ROS (Reactive oxygen species) responsive rapamycin liposome, and to develop a stable and efficient stimulus-responsive liposome carrier. Methods Rapamycin liposomes were prepared by thin film dispersion method. The particle size and Zeta potential were determined by Malvern laser particle size analyzer. An assay method for rapamycin was established by HPLC. In vitro release characteristics of rapamycin liposomes were investigated by reverse dialysis after irradiation with near-infrared light. Results The particle size of rapamycin liposome was less than 200 nm and the PDI value was less than 0.200. Rapamycin showed a good linear relationship with peak area in the range of 0.2-40 μg/ml, with the correlation coefficient of 0.9995. Encapsulation rate of rapamycin liposomes was > 94.20%. The release efficiency of rapamycin liposomes reached 60% within 12 h after irradiation with 730 nm near infrared light for 5 min. Conclusion Photosensitive ROS-responsive rapamycin liposomes were successfully prepared, which had high encapsulation rate and stimulation response efficiency in vitro.
