Protective effect of Saussurea involucrata flavone capsule on myocardial injury induced by hypoxia at high altitude in mice
10.12206/j.issn.2097-2024.202205086
- VernacularTitle:雪莲黄酮胶囊对高原缺氧诱导小鼠心肌损伤的保护作用研究
- Author:
Pengpeng ZHANG
1
,
2
;
Lin LI
3
;
ZhiQun SHI
3
;
Lei HE
3
;
Huiping MA
1
,
2
Author Information
1. Key Laboratory of the Plateau Medicine, the 940th Hospital of Joint Logistics Support Force of the PLA, Lanzhou 730050, China
2. School of Pharmacy, Gansu University of Chinese Medicine, Lanzhou 730000, China.
3. Key Laboratory of the Plateau Medicine, the 940th Hospital of Joint Logistics Support Force of the PLA, Lanzhou 730050, China.
- Keywords:
Saussurea involucrata;
plateau;
anti-hypoxia;
hypoxia-inducible factor 1α;
epidermal growth factor
- From:
Journal of Pharmaceutical Practice and Service
2022;40(5):403-407
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protective effect and mechanism of Saussurea involucrata flavone capsule on the myocardial tissue of mice in simulated plateau hypoxia environment. Methods 64 Balb/c mice were randomly divided into normal control group, hypoxia model group, positive control group and Xuelian flavonoid capsule group. Myocardial tissue was observed microscopically and ultra-structurally, and the changes of hypoxia-related indexes were detected. The changes in the transcription levels of hypoxia-related genes were detected by RT-PCR, and the changes in the protein expressions of hypoxia-related genes were detected by Western blotting to study the mechanism of action of Xuelian flavone capsules. Results Saussurea involucrata flavone capsule had significantly alleviated the pathological damage to the myocardium of mice caused by simulated altitude hypoxia, increased the activity of T-AOC in myocardial tissue, reduced the accumulation of LD, and also reduced the expression levels of HIF-1α and VEGF mRNA in myocardial tissue, increased the SOD, CAT mRNA and protein expression levels. Conclusion Saussurea involucrata flavone capsule have good anti-altitude hypoxia effect, which could protect myocardial tissue structure and function, regulate energy metabolism, and improve antioxidant capacity. The mechanism might be related to improving the antioxidant capacity, regulating energy metabolism, and affecting the protein expression of HIF-1α, VEGF, SOD and CAT mRNA in high altitude hypoxic mice.
