Systematic Review of Chinese Medicine in Improving Sperm Quality in Animal Experiments Based on Structure and Function of Sertoli Cells
10.13422/j.cnki.syfjx.20221411
- VernacularTitle:基于睾丸支持细胞结构功能探讨中药改善生精功能的动物实验系统评价
- Author:
Yifeng SHEN
1
;
Kun ZHU
1
;
Wenyuan LI
1
;
Liang DONG
2
;
Yaodong YOU
1
;
Degui CHANG
1
;
Xujun YU
3
Author Information
1. Key Laboratory of Traditional Chinese Medicine（TCM） in Regulation of Metabolic Diseases in Sichuan Province，Hospital of Chengdu University of TCM， Chengdu 610075，China
2. Reproductive ＆ Women-Children Hospital，Chengdu University of TCM，Chengdu 610041，China
3. School of Medical and Life Sciences，Chengdu University of TCM，Chengdu 610075，China
- Publication Type:Journal Article
- Keywords:
Chinese medicine;
Sertoli cells;
spermatogenic function;
Meta-analysis;
systematic review
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(21):201-211
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo systematically review the intervention effect of Chinese medicine on the structure and function of testicular Sertoli cells in animal models of impaired spermatogenesis. MethodThe databases， such as China National Knowledge Infrastructure （CNKI），VIP，Wanfang Data，EMbase，and Pubmed，were searched for experimental studies on the effect of Chinese medicine on the structure and function of testicular Sertoli cells in animal models with impaired spermatogenesis. The included studies were evaluated for risks of bias，and the outcome indicators were analyzed with RevMan and Stata software. ResultThirty studies were included，involving 37 randomized controlled trials （RCTs）. As indicated by the Meta-analysis results， compared with the model group，Chinese medicine increased sperm density（SMD=2.42，95% confidence interval（CI）［1.47，3.37］，P<0.000 01）， promoted sperm motility（SMD=2.35，95%CI ［1.70， 2.99］，P<0.000 01）， up-regulated the protein and mRNA levels of Vimentin （related to Sertoli cell cytoskeleton）， elevated the levels of Occludin and Claudin-11 （related to tight junction of blood-testis barrier）， boosted the levels of β-catenin and N-cadherin （related to adherens junction of blood-testis barrier）， raised the level of connexin 43 （Cx43， related to gap junction of blood-testis barrier）， improved the function of Sertoli cells， increased the serum content of Inhibin B （INHB）， and up-regulated the levels of testicular follicle-stimulating hormone receptor （FSHR）， INHB mRNA， androgen-binding protein （ABP） mRNA， transferrin（TF），stem cell factor（SCF），SCF mRNA，glial cell line-derived neurotrophic factor （GDNF），GDNF mRNA，bone morphogenetic protein 4（BMP4），and BMP4 mRNA （P<0.05）. ConclusionChinese medicine can effectively increase sperm density and motility of animal models of impaired spermatogenesis，and improve the structure and function of testicular Sertoli cells. However，affected by the quality of the included studies，the above conclusion needs to be further verified by relevant high-quality studies.
