Metabolic Changes of Acanthopanax senticosus Fruits During Development
10.13422/j.cnki.syfjx.20221216
- VernacularTitle:刺五加果实发育过程的代谢差异
- Author:
Feiyang SUN
1
;
Xiaorui GUO
1
;
Ying SONG
1
;
Mingyuan XU
2
;
Jing ZHANG
3
;
Zhonghua TANG
1
;
Hongwei NI
2
Author Information
1. College of Chemistry，Chemical Engineering and Resource Utilization，Northeast Forestry University，Harbin 150040，China
2. Heilongjiang Academy of Forestry，Harbin 150040，China
3. School of Clinical Medicine，Heilongjiang University of Chinese Medicine，Harbin 150040，China
- Publication Type:Journal Article
- Keywords:
Acanthopanax senticosus fruit;
metabolomics;
multivariate statistical analysis;
gas chromatography-mass spectrometry （GC-MS）;
liquid chromatography-mass spectrometry （LC-MS）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(21):163-170
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the changes of primary metabolites and phenols in the fruits of Acanthopanax senticosus at different development stages, so as to provide a theoretical basis for the rational utilization of A. senticosus fruit resources. MethodThe primary metabolites and phenols in the fruits at different development stages were determined via gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) and then compared by multivariate statistical analysis. ResultA total of 274 chromatographic peaks were obtained by GC-MS-based non-targeted metabonomics and 24 differential metabolites were screened out by multivariate statistical analysis. The differential metabolites were mainly concentrated in pentose phosphate pathway, galactose metabolism, ascorbic acid and aldose metabolism pathways. After color conversion, the pentose phosphate pathway and galactose metabolism were activated and increasing sugars were accumulated. The ascorbic acid and aldose metabolism pathways were active before color conversion, with high accumulation of the end product ascorbic acid. The ultra-high liquid chromatography-mass spectrometry (UPLC-MS) identified 28 phenols in the fruits at different development stages. Flavonoids were accumulated mainly at the green ripening stage before color conversion, and phenolic acids were accumulated mainly after color conversion. ConclusionThe accumulation of primary metabolites and phenols in A. senticosus fruits varies significantly among different development stages
