Effect of Dahuang Xiezhuo Prescription on Renal Interstitial Fibrosis of Rats with 5/6 Nephrectomy by Regulating ROS/TXNIP/NLRP3 Pathway
10.13422/j.cnki.syfjx.20221944
- VernacularTitle:大黄泄浊方调控ROS/TXNIP/NLRP3通路对5/6肾切除大鼠肾间质纤维化的影响
- Author:
Wenxuan CAO
1
;
Xin XIN
1
;
Miao TAN
2
;
Fei GAO
3
;
Fengwen YANG
4
;
Suzhi CHEN
4
;
Meifang REN
4
;
Jinchuan TAN
4
Author Information
1. Hebei University of Chinese Medicine， Shijiazhuang 050200， China
2. The Fourth Hospital of Hebei Medical University， Shijiazhuang 050000， China
3. Dongzhimen Hospital， Beijing University of Chinese Medicine， Beijing 100700， China
4. Hebei Provincial Hospital of Traditional Chinese Medicine， Shijiazhuang 050011， China
- Publication Type:Journal Article
- Keywords:
Dahuang Xiezhuo prescription;
chronic renal failure;
renal interstitial fibrosis;
oxidative strss;
thioredoxin-interacting protein （TXNIP）;
NOD-like receptor protein 3 （NLRP3）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(21):81-89
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the effect of Dahuang Xiezhuo prescription on the changes in renal pathology and reactive oxygen species （ROS）/thioredoxin-interacting protein （TXNIP）/NOD-like receptor protein 3 （NLRP3） pathway expression in the kidney tissues of rats with 5/6 nephrectomy， and to explore the mechanism of Dahuang Xiezhuo prescription in protecting renal function and delaying renal interstitial fibrosis and the possibility. MethodNinety healthy male SD rats were randomly divided into a sham operation group， a model group， low， medium， and high-dose （6.825， 13.65， 27.30 g·kg-1） Dahuang Xiezhuo prescription groups， and a Niaoduqing granule group （2.60 g·kg-1）. Except the sham operation group， 5/6 nephrectomy was used to replicate the rat model of chronic renal failure （CRF）. After modeling， each administration group was given the corresponding dose of drug suspension by intragastric administration， once a day for consecutive 8 weeks. After administration， serum creatinine （SCr） and urea nitrogen （BUN） levels and 24 h urinary protein quantification （UTP） levels were detected. Western blot assay was used to detect the protein expressions of thioredoxin （TRX）， TXNIP， and NLRP3. The protein expressions of TRX， TXNIP， NLRP3， apoptosis-associated speck-like protein containing a CARD （ASC）， transformation growth factor-β （TGF-β）， Collagen Ⅳ， α-smooth muscle actin （α-SMA）， and fibronectin （FN） were detected by immunohistochemistry. ResultAs compared with the sham operation group， serum levels of SCr， BUN， and UTP in the model group were increased （P<0.05）， TRX， TXNIP， NLRP3， ASC， TGF-β， Collagen Ⅳ， α-SMA， and FN proteins were increased （P<0.01）， and renal interstitial fibrosis significantly occurred. As compared with the model group， the levels of SCr， 24 h BUN， and UTP in the low， medium， and high-dose Dahuang Xiezhuo prescription groups and the Niaoduqing granule group were decreased to varying degrees （P<0.05）， TRX， TXNIP， NLRP3， ASC， TGF-β， Collagen Ⅳ， α-SMA， and FN were decreased （P<0.01）， and renal interstitial fibrosis was improved to varying degrees. ConclusionDahuang Xiezhuo prescription can protect renal function and delay renal interstitial fibrosis in rats with CRF.
