The Effects of Amniotic Membrane Contact Lens for Cornea Wound Healing.
10.3341/jkos.2009.50.7.989
- Author:
Jang Won SEO
1
;
Byeong Wook KO
;
Dong Jun LEE
;
Woo Chan PARK
Author Information
1. Department of Ophthalmology, College of Medicine, Dong-A University, Busan, Korea. wcpark@daunet.donga.ac.kr
- Publication Type:Original Article
- Keywords:
Amniotic membrane;
Contact lens;
Wound healing
- MeSH:
Amnion;
Contact Lenses;
Cornea;
Eye;
Fluorescein;
In Situ Nick-End Labeling;
Membranes;
Rabbits;
Sutures;
Transplants;
Wound Healing
- From:Journal of the Korean Ophthalmological Society
2009;50(7):989-995
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate the efficacy of an amniotic membrane contact lens on corneal epithelial wound healing. METHODS: We made a model with a corneal epithelial wound by applying 6 mm round filter paper soaked with 1 N NaOHonto the central cornea in 24 eyes of 12 rabbits. The rabbits were divided into three groups: AMCL (amniotic membrane contact lens), T-AMT (temporary amniotic membrane transplantation) and the control group. We evaluated corneal wound healing every postoperative day using a digital photo slitlamp and fluorescein dye. The corneas were harvested for histopathologic studies after seven days and analyzed with hematoxylin-eosin (H & E) stain and TUNEL staining. RESULTS: The average wound healing time was similar between the amniotic membrane contact lens and the temporary amniotic membrane transplantation group. The number of the infiltrated PMNs (polymorphonuclear cells) was 8.8+/-2.58, 8.6+/-2.19 and 48.6+/-7.12 in the AMCL, T-AMT and control groups, respectively. Apoptotic keratocytes were 3.8+/-1.1, 3.6+/-1.09 and 23.2+/-5.06 in the AMCL, T-AMT and control groups, respectively. In the AMCL and T-AMT groups, the number of infiltrated PMNs and apoptotic keratocytes were significantly less than those the control group (p<0.05). There were not significant differences in the number of PMNs and apoptotic cells in the AMCL and the T-AMT groups. CONCLUSIONS: Amniotic membrane contact lenses have the benefits of being an easily applied method and having a wound healing ability comparable to that possible with conventional suture methods.
