Effect of Huatan Qushi Huoxue prescription on lipopolysaccharide-induced pyroptosis of macrophages
10.3969/j.issn.1001-5256.2022.09.014
- VernacularTitle:化痰祛湿活血方对脂多糖诱导的巨噬细胞焦亡形态的影响
- Author:
Minghao LIU
1
;
Sutong LIU
1
;
Dongfang SHANG
1
;
Lihui ZHANG
1
;
Yajiao GU
1
;
Wenxia ZHAO
1
Author Information
1. Department of Hepatology and Spleen-Stomach, The First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou 450000, China
- Publication Type:Original Articles_Fatty Liver Diseases
- Keywords:
Fatty Liver;
Macrophages;
Pyroptosis
- From:
Journal of Clinical Hepatology
2022;38(9):2016-2019
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Huatan Qushi Huoxue prescription on lipopolysaccharide (LPS)-induced pyroptosis of RAW264.7 cells and its mechanism. Methods An in vitro cell model of LPS-induced activated RAW264.7 was established and divided into blank group, model group, high-, middle-, and low-dose Huatan Qushi Huoxue prescription groups, and control group. The corresponding drug-containing serum intervention was performed for 24 hours. A scanning electron microscope was used to observe cell morphology, and immunofluorescence assay was used to perform quantitative localization of GSDMD-N. Results The cells in the blank group were round and regular in shape with smooth surface, and those in the control group were swollen, with folds on the surface and gaps in the capsule, which were consistent with the morphology of cell pyroptosis. The cells in the control group had bubbles on the surface with obvious pseudopodia and pores in cell membrane, and those in the high-dose group were not swollen and had a rough surface with pseudopodia, with no obvious pores in cell membrane. The cells in the low- and middle-dose groups were swollen and had a rough surface of cell membrane with pores and pseudopodia. Immunofluorescence assay showed that compared with the blank group, the model group had a significant increase in the positive staining intensity of GSDMD-N, and compared with the model group, the control group and the Traditional Chinese medicine group had a reduction in the positive staining intensity of GSDMD-N. Conclusion Huatan Qushi Huoxue prescription can improve the pyroptosis of macrophages and reduce the expression of GSDMD-N.
