Mechanism of Jiawei Bazhen Yimu Capsule in Treatment of Premature Ovarian Failure Based on Estrogen Signaling Pathway
10.13422/j.cnki.syfjx.20221414
- VernacularTitle:基于雌激素信号通路传导加味八珍益母胶囊治疗卵巢早衰的作用机制
- Author:
Liying HAN
1
;
Shuai WANG
1
;
Yongrui BAO
1
;
Tianjiao LI
1
;
Lingwei ZHANG
2
;
Xiansheng MENG
1
Author Information
1. College of Traditional Chinese Medicine（TCM）， Liaoning University of TCM， Dalian 116600，China
2. Dandong Pharmaceutical Group Co. Ltd.， Dandong 118303，China
- Publication Type:Journal Article
- Keywords:
Jiawei Bazhen Yimu capsule;
premature ovarian failure;
ovariectomy;
estrogen signaling pathway
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(20):201-208
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect of Jiawei Bazhen Yimu capsule on serum sex hormones， sexual organs and estrogen signaling pathway in female rats with premature ovarian failure. MethodThe key target proteins of Jiawei Bazhen Yimu capsule in the treatment of premature ovarian failure were screened out by network pharmacology analysis. Female healthy SD rats were selected, and the rat model of premature ovarian failure was established by ovariectomy. Fifty ovariectomized rats were randomly divided into a model group, an estradiol (E2) valerate group, and Jiawei Bazhen Yimu capsule low, medium, and high-dose groups. Another 10 healthy female rats were set as a sham operation group. The sham operation group and the model group were given distilled water by gavage, and other administration groups were given corresponding doses of drugs by gavage. After 21 d, the serum hormone levels of female rats were measured, including E2, progesterone (P), follicle stimulating hormone (FSH), and luteinizing hormone (LH). Immunofluorescence staining (IF) was used to detect the protein expression levels of estrogen receptor 1 (ESR1), estrogen metabolism P4503A4 enzyme (CYP3A4), and P45019A1 enzyme (CYP19A1) in the uterine tissues of female rats. ResultAs compared with the model group, the serum E2 and P levels of female rats in the Jiawei Bazhen Yimu capsule low, medium, and high-dose groups were significantly increased. Jiawei Bazhen Yimu capsule improved the endometrial status of female rats and increased positive expression of ESR1, CYP3A4, and CYP19A1 in the uterine tissues of female rats (P<0.05). ConclusionThe mechanism of Jiawei Bazhenyimu capsule in the treatment of premature ovarian failure may be related to its hormone-like effect and activation of the estrogen signaling pathway.
