Effect of subchronic combined oral exposure of titanium dioxide nanoparticles and glucose on levels of serum folate and vitamin B12 in young SD rats.
10.19723/j.issn.1671-167X.2020.03.009
- Author:
Zhang Jian CHEN
1
;
Shuo HAN
1
;
Pai ZHENG
1
;
Shu Pei ZHOU
2
;
Guang JIA
1
Author Information
1. Department of Occupational and Enviromental Health Sciences, Peking University School of Public Health, Beijing 100191, China.
2. Department of Laboratory Animal Science, Peking University Health Science Center, Beijing 100191, China.
- Publication Type:Journal Article
- Keywords:
Folic acid;
Glucose;
Rats, Sprague-Dawley;
Titanium dioxide nanoparticles;
Vitamin B12
- MeSH:
Animals;
Female;
Folic Acid;
Glucose;
Male;
Metal Nanoparticles;
Rats;
Rats, Sprague-Dawley;
Titanium;
Vitamin B 12;
Vitamins
- From:
Journal of Peking University(Health Sciences)
2020;52(3):451-456
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effect of subchronic combined oral exposure of titanium dioxide nanoparticles and glucose on levels of serum folate and vitamin B12 in young SD rats.
METHODS:At first, the physical and chemical properties of titanium dioxide nanoparticles, such as particle size, shape, crystal form and agglomeration degree in solution system, were characterized in detail. Eighty 4-week-old young SD rats were randomly divided into 8 groups (10 rats in each group, half male and half female). The rats were exposed to titanium dioxide nanoparticles through intragastric administration at 0, 2, 10 and 50 mg/kg body weight with or without 1.8 g/kg glucose daily for 90 days. At last, the concentrations of serum folate and vitamin B12 were detected.
RESULTS:Titanium dioxide nanoparticles were anatase crystals, closely spherical shape, with an average particle size of (24±5) nm. In male young rats, compared with the control group, the serum folate concentration was significantly increased when exposed to titanium dioxide nanoparticles (10 mg/kg) and glucose. The difference was statistically significant (P<0.05). However, in female and male young rats, compared with glucose (1.8 g/kg) exposure group, titanium dioxide nanoparticles (50 mg/kg) and glucose significantly reduced the serum folate concentration. The difference was statistically significant (P<0.05). Through statistical analysis of factorial design and calculation of interaction, obvious antagonistic effect was observed between titanium dioxide nanoparticles and glucose on the serum folate concentration in the young female SD rats. The combined oral exposure of titanium dioxide nanoparticles and glucose had little effect on the concentration of serum vitamin B12 in the young SD rats, with no significant interaction between the two substances. It was only found that titanium dioxide nanoparticles (2 mg/kg) and glucose significantly increased the serum vitamin B12 concentration, compared with glucose (1.8 g/kg) exposure group. The difference was statistically significant (P<0.05).
CONCLUSION:Subchronic combined oral exposure of titanium dioxide nanoparticles and glucose had an obvious antagonistic effect on serum folate concentrations in young SD rats.
