Effect of Cluster Needling of Scalp Acupuncture Combined with Rehabilitation on Neurological Function and Expression of Basic Fibroblast Growth Factor and Angiostatin in Rats after Focal Cerebral Ischemia
10.3969/j.issn.1006-9771.2015.10.007
- VernacularTitle:针康法对局灶性脑缺血大鼠神经功能缺损及缺血区碱性成纤维细胞生长因子、血管抑制素蛋白表达的影响
- Author:
Qiang TANG
;
Yunfei JIANG
;
Luwen ZHU
;
Tao YE
;
Mingyang SONG
- Publication Type:Journal Article
- Keywords:
cerebral ischemia, neurological function, acupuncture and rehabilitation therapy, basic fibroblast growth factor, angiostatin, rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2015;21(10):1151-1155
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of cluster needling of scalp acupuncture combined with rehabilitation on neurological function and expression of basic fibroblast growth factor (bFGF) and angiostatin (AS) in rats after focal cerebral ischemia. Methods 90 male Sprague-Dawley rats were randomly divided into sham operation group, model group, acupuncture treatment group, rehabilitation treatment group and acupuncture and rehabilitation treatment group, each group was further divided into 3 days, 7 days and 14 days subgroups (n=6). Permanent cerebral ischemia rat model was established according to Longa's method. The model group and the sham operation group accepted no treatment, the acupuncture treatment group was treated by cluster needling of scalp acupuncture, the rehabilitation treatment group received the treadmill training, the acupuncture and rehabilitation treatment group was treated by acupuncture and rehabilitation therapy. 3 days, 7 days, 14 days after operation, the neural function was evaluated with the modified Neurological Severity Score (mNSS), the expression levels of bFGF and AS proteins were detected by Western blotting. Results Compared with the model group, the acupuncture treatment group and the rehabilitation treatment group, mNSS decreased, the expression of bFGF protein increased, and the expression of AS protein decreased in the acupuncture and rehabilitation treatment group 3 days, 7 days, 14 days after operation (P<0.05). Conclusion Acupuncture and rehabilitation therapy can reduce the neurological function defect in rats with focal cerebral ischemia, which may be related to the upregulation of bFGF protein expression and down-regulation of AS protein expression.
