Analysis of flavonoids and phenylethanoid glycosides in the Tibetan herb Lagotis brevituba Maxim based on UHPLC-LTQ-orbitrap-MS
10.16438/j.0513-4870.2022-0504
- VernacularTitle:基于UHPLC-LTQ-orbitrap-MS方法分析藏药洪连 (短管兔耳草) 中黄酮与苯乙醇苷类成分
- Author:
Zhao GENG
1
,
2
;
Bi-xing GAO
1
,
3
;
Lian ZHONG
1
,
3
;
Jing-liang QI
4
;
Yan GOU
1
;
Yun-bin JIANG
5
;
Lei YANG
1
;
Jun YUAN
1
;
Li GUO
4
;
Yi-tao WANG
6
Author Information
1. NMPA Key Laboratory for Quality Evaluation of Traditional Chinese Medicine (Traditional Chinese Patent Medicine), Sichuan Institute for Drug Control (Sichuan Testing Center of Medical Devices), Chengdu 611731, China
2. State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau 999078, China
3. College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China
4. College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China
5. College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China
6. State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau 999078, China
- Publication Type:Research Article
- Keywords:
italic>Lagotis brevituba Maxim.;
UHPLC-LTQ-orbitrap-MS;
flavonoid;
phenylethanoid glycoside;
italic>Lagotis genus
- From:
Acta Pharmaceutica Sinica
2022;57(9):2821-2838
- CountryChina
- Language:Chinese
-
Abstract:
Ultra high performance liquid chromatography tandem linear ion trap orbitrap mass spectrometry (UHPLC-LTQ-orbitrap-MS) was applied to analyze and identify flavonoids and phenylethanoid glycosides in the Tibetan herb Lagotis brevituba Maxim. A method of data-dependent scan coupling with dynamic exclusion was developed for analyzing flavonoids and phenylethanoid glycosides under positive and negative ion mode of electrospray ionization (ESI). The compounds of Lagotis brevituba Maxim. were systematically identified through exact molecular mass, fragmentation patterns, retention time and reported references. A total of 167 compounds were detected, of which 84 were flavonoids and 83 were phenylethanoid glycosides, which greatly enriched the number and types of flavonoids and phenylethanol glycosides in Lagotis genus medicinal plants. Baohuoside Ⅰ, 4 disaccharide O-glycoside flavonoids (composed of deoxyhexose and glucuronic acid), 9 C-glycoside flavonoids, 15 tetrasaccharide phenylethanoid glycosides and 5 phenylethanoid glycosides with substituents on the β-position of the phenylethyl group were identified in Lagotis genus medicinal plants for the first time. This study provides scientific support for elucidating the material basis and improving the quality control of Lagotis brevituba Maxim.
