Metabolites of schaftoside in mouse plasma, bile, urine and feces after oral administration by UPLC-Q/TOF-MS/MS
10.16438/j.0513-4870.2022-0436
- VernacularTitle:UPLC-Q/TOF-MS/MS法分析鉴定夏佛塔苷在小鼠体内的代谢产物
- Author:
Ya-qin WEI
1
;
Tian-yun WANG
2
;
Ya-ting LI
1
;
Yu-lang CHEN
1
,
3
;
Wen-xing LIAO
1
;
Yan DU
1
;
Dao-quan TANG
1
Author Information
1. Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou 221004, China
2. Huai'an Hosipital of Huai'an City, Huai'an 223200, China
3. Huai'an Hosipital of Huai'an City, Huai'an 223200, China
- Publication Type:Research Article
- Keywords:
schaftoside;
mouse;
parent compound;
metabolite;
UPLC-Q/TOF-MS/MS
- From:
Acta Pharmaceutica Sinica
2022;57(9):2811-2820
- CountryChina
- Language:Chinese
-
Abstract:
Ultra-high performance liquid chromatography coupled with quadrupole/time-of-flight tandem mass spectrometry (UPLC-Q/TOF-MS/MS) has been used to detect the metabolites of schaftoside in plasma, bile, urine and feces of mice after oral administration. The study was approved by the Experimental Animal Ethics Committee from Xuzhou Medical University (No. XZMULL201612024). Compounds were identified by analyzing their high-resolution mass spectrometry data, mass spectra, and comparison with reference substances and the literatures. The parent compound and 29 metabolites were detected in the plasma, bile, urine and feces samples of mice. The main metabolic pathways of schaftoside in mice include deglycosylation/glycosylation, hydroxylation/dehydroxylation, hydrogenation, methylation, acetylation, sulfation, and glucuronidation. This study provides references for the material basis of schaftoside in vivo.
