Bioinformatics and expressional analysis of WRKY transcription factor family in Baphicacanthus cusia
10.16438/j.0513-4870.2022-0433
- VernacularTitle:马蓝WRKY转录因子家族生物信息学及表达特征分析
- Author:
Zhi-ying GUO
1
,
2
;
Qing LI
3
;
Xun-xun WU
4
;
Jun-feng CHEN
5
;
Yu-xiang HUANG
6
;
Xiao-juan MA
7
;
Yong DIAO
4
;
Lei ZHANG
8
Author Information
1. School of Food and Bioengineering, Fujian Polytechnic Normal University, Fuqing 350300, China
2. School of Pharmacy, Navy Medical University, Shanghai 200433, China
3. Department of Pharmacy, Second Affiliated Hospital of Naval Medical University, Shanghai 200003, China
4. School of Medicine, Huaqiao University, Quanzhou 362021, China
5. Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
6. School of Pharmacy, Quanzhou Medical College, Quanzhou 362000, China
7. Quanzhou Institute of Agricultural Sciences, Quanzhou 362212, China
8. School of Pharmacy, Navy Medical University, Shanghai 200433, China
- Publication Type:Research Article
- Keywords:
italic>Baphicacanthus cusia;
WRKY transcription factor;
bioinformatics;
expression analysis;
indole alkaloid
- From:
Acta Pharmaceutica Sinica
2022;57(9):2864-2875
- CountryChina
- Language:Chinese
-
Abstract:
WRKY, a class of conserved transcription factors in plants, plays important roles in plant growth, development and secondary metabolism. In the present study, 65 WRKY members were identified from de novo transcriptome sequencing data of three different tissues (root, stems and leaves) of Baphicacanthus cusia. BcWRKY proteins contained from 221 to 706 amino acids and the isoelectric point is from 4.68 to 9.68. Molecular weights range from 25 711.8 to 75 475 Da. The main secondary structures of BcWRKYs protein are random coil. A subcellular localization prediction indicated that the putative BcWRKY proteins were enriched in the nuclear region. Phylogenetic analysis showed that BcWRKYs could be categorized into three groups and five subgroups (Group IIa, Group IIb, Group IIc, Group IId and Group IIe) in Group II. Structural analysis found that all BcWRKY proteins contained a highly conserved motif WRKYGQK. Finally, the transcriptional profiles of ten BcWRKY genes highly expressed in root, stem and leaf tissues under abscisic acid (ABA), methyl jasmonate (MeJA), or salicylic acid (SA) treatment were systematically investigated using qRT-PCR analysis. Results showed that a total of ten BcWRKY genes were differentially expressed in response to ABA, MeJA, and SA treatment. This work would be provided a basis for further elucidating the molecular mechanism of WRKY transcription factors in the biosynthesis of indole alkaloids in B. cusia.
