Q-marker analysis of Kanggongyan soft capsule
- VernacularTitle:抗宫炎软胶囊的质量标志物分析
- Author:
Minyan YUAN
1
,
2
;
Min ZHANG
1
;
Shuo ZHANG
3
;
Siyuan CAO
1
,
2
;
Jiacheng JI
1
,
2
;
Pengjiao WANG
1
;
Rongping ZHANG
4
;
Xiuli GAO
1
Author Information
1. State Key Laboratory of Function and Application of Medicinal Plants/School of Pharmacy，Guizhou Medical University，Guiyang 550025，China
2. Center for Microbiology and Biochemical Pharmaceutical Engineering，Guiyang 550025，China
3. Experimental Animal Center，Guizhou Medical University，Guiyang 550025，China
4. School of Pharmacy，Kunming Medical University，Kunming 650500，China
- Publication Type:Journal Article
- Keywords:
Kanggongyan soft capsule;
network pharma-
- From:
China Pharmacy
2022;33(17):2082-2086
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To analyze quality maker （Q-marker） of Ka nggongyan soft capsule （KSC）. METHODS The fingerprints of 20 batches of KSC were established by ultra high performance liquid chromatography （UPLC）method. Similarity Evaluation System of TCM Chromatographic Fingerprint （2012 edition）were used to evaluate the similarity and confirm common peaks. The contents of norisoboldine ，leonurine hydrochloride ，forsythoside B ，acteoside，poliumoside and isoacteoside were determined by the same UPLC method. Targets and pathways related to KSC in the treatment of cervicitis were screened and analyzed by network pharmacology and molecular docking method to construct a “component-target-pathway”network，and analyze its potential Q-marker. RESULTS Twelve common peaks were identified in the fingerprints of 20 batches of KSC ，and the similarity was greater than 0.99. Six common peaks were identified ，including norisoboldine ，leonurine hydrochloride ，forsythoside B，acteoside，poliumoside and isoacteoside. The contents of the above 6 components were 1.336-1.774，0.093-0.143，4.970-5.888， 0.505-0.623，5.206-6.226 and 0.785-0.895 mg/g，respectively. By network pharmacology analysis ，14 key targets and 94 pathways were obtained ，and their binding energies to the core targets （protein kinase B 1，tumor necrosis factor ）were all less than －6.4 kJ/cal. CONCLUSIONS Six components such as norisoboldine and leonurine hydrochloride are potential Q-marker of KSC.
