Fingerprint establishment of Qingyi mixture and content determination of 8 components
- VernacularTitle:清胰合剂的指纹图谱建立及8种成分含量测定
- Author:
Fanfan ZHANG
1
;
Zheyuan WANG
1
;
Longlong WANG
1
;
Hengwen CHEN
2
;
Ju LIU
3
;
Xia HUANG
4
;
Xiaotao YU
1
;
Rui WANG
1
Author Information
1. Dept. of Pharmacy，Luohe Central Hospital，Henan Luohe 462300，China
2. Dept. of Pharmacy，Guang’anmen Hospital，China Academy of Chinese Medical Sciences，Beijing 100053，China
3. Dept. of Pharmacy，Zhengzhou Hospital of Traditional Chinese Medicine，Zhengzhou 450007，China
4. Laboratory of Traditional Chinese Medicine Research，Henan Provincial Institute for Food and Drug Control，Zhengzhou 450003，China
- Publication Type:Journal Article
- Keywords:
Qingyi mixture;
fingerprint;
chemical pattern
- From:
China Pharmacy
2022;33(17):2077-2081
- CountryChina
- Language:Chinese
-
Abstract:
OBJECT IVE To provide scientific evidence for the quality standard research of Qingyi mixture （QM）qualitatively and quantitatively. METHODS The high performance liquid chromatography （HPLC）fingerprint of QM was established ，and the chemical pattern recognition analysis was carried out. At the same time ，the contents of 8 components such as chlorogenic acid in the preparation were determined. The determination was performed on Agilent SB-C 18 column with 0.1％ phosphoric acid-acetonitrile as mobile phase （gradient elution ）at the flow rate of 0.6 mL/min. The column temperature was 35 ℃，and detection wavelength was set at 254 nm. Similarity Evaluation System of Chromatographic Fingerprint of Traditional Chinese Medicine（2012 edition），SPSS 20.0 and SIMCA 14.1 were used to perform similarity evaluation ，cluster analysis （CA），principle component analysis （PCA）and orthogonal partial least squares-discriminant analysis （OPLS-DA）of QM samples. RESULTS A total of 22 common peaks were calibrated by 15 batches of QM ，and the similarity was over 0.975. Twenty-two common peaks were assigned and 8 of them were identified. CA ，PCA and OPLS-DA divided the 15 batches of QM into two categories. Meanwhile，5 differential components were screened out ，i.e. peak 9（cichoric acid ），peak 14（baicalin），peak 18，peak 19 and peak 21 （baicalein）. The contents of 8 components，such as chlorogenic acid ，ferulic acid ，cichoric acid ，hesperidin， baicalin，salvianolic acid B ，baicalein and paeonol ，were 0.077-0.094，0.165-0.190，0.100-0.114，0.083-0.107，0.556-0.615，0.288-0.314，0.152-0.188 and 0.114-0.128 mg/g，respectively. CONCLUSIONS The established HPLC fingerprint and content determination method can provide reference for the quality standard research of QM.
