Expression of tropomyosin 2 in aortic dissection tissue.
10.3760/cma.j.cn112148-20200707-00540
- VernacularTitle:原肌球蛋白2表达变化对主动脉夹层影响的实验研究
- Author:
Xiao Xuan ZHONG
1
;
Xiang WEI
1
;
Ding Sheng JIANG
1
;
Xue Hai ZHU
1
;
Li Gang LIU
1
Author Information
1. Divison of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
- Publication Type:Journal Article
- Keywords:
Aortic dissection;
Aortic smooth muscle cells;
Tropomyosin 2
- MeSH:
Aneurysm, Dissecting/genetics*;
Aorta;
Aortic Aneurysm, Thoracic/genetics*;
Gene Expression;
Humans;
RNA, Messenger;
Tropomyosin/metabolism*
- From:
Chinese Journal of Cardiology
2020;48(9):777-781
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the expression pattern of tropomyosin 2(TPM2) in aorta of patients with aortic dissection and explore its clinical implication. Methods: Thirteen cases with acute type A aortic dissection(TAAD) diagnosed by transabdominal aortic angiography from 2015 in Tongji Hospital were included. During the operation, the aortic wall tissues of these patients were collected. Ten patients with heart transplantation were selected as control group, and normal aortic wall tissues were taken. The hematoxylin-eosin (HE) and Verhoeff's Van Gieson (EVG) staining were performed to observe the morphological changes of aorta. The mRNA expression level of TPM2 was measured by real-time fluorescent quantitative-PCR, and the protein levels of TPM2 were detected by Western blot and immunohistochemical staining. Image The J software was used to collect the optical density values of each point on the image, obtain the integrated optical density(IOD) value, and calculate the average density(%, IOD/area of the target distribution area). Results: HE and EVG staining revealed medial degeneration and broken elastic fiber in aorta of TAAD patients. The mRNA expression levels of TPM2 were significantly upregulated in aorta of TAAD patients as compared to the control group (P<0.05), so as the TPM2 protein expression levels ((9.73±1.20)% vs. (0.11±0.04)%, P<0.05). And TPM2 was mainly expressed in cytoplasm. Conclusion: The increased expression of TPM2 in TAAD patients hints that TPM2 might be involved in the pathogenesis of aortic dissection.
