Juglone induces proliferation inhibition and apoptosis of cervical cancer cells via promoting c-Myc ubiquitination.
10.12122/j.issn.1673-4254.2022.07.09
- Author:
Xing Yu ZHAO
1
;
Kun YANG
1
;
Zi Tong SONG
1
;
Han HE
2
;
Wei ZHANG
1
Author Information
1. Department of Biochemistry, Jilin Medical College, Jilin 132013, China.
2. Department of Biochemistry, School of Basic Medicine, Yanbian University, Yanbian 133000, China.
- Publication Type:Journal Article
- Keywords:
HeLa cells;
c-Myc;
cervical cancer;
juglone;
ubiquitination
- MeSH:
Apoptosis;
Cell Proliferation;
Female;
HeLa Cells;
Humans;
Naphthoquinones;
Ubiquitination;
Uterine Cervical Neoplasms/genetics*
- From:
Journal of Southern Medical University
2022;42(7):1026-1031
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the expression of c-Myc protein in cervical cancer HeLa cells and explore the effect of juglone on the proliferation and apoptosis of HeLa cells by affecting c-Myc ubiquitination.
METHODS:HeLa cells treated with different concentrations (0, 10, 20, or 50 μmol/L) of juglone or with 20 μmol/L juglone for different time lengths were examined for expression of c-Myc protein with Western blotting. The half-life of c-Myc protein was determined using cycloheximide (CHX) and c-Myc protein degradation was detected using coimmunoprecipitation. We also assessed the effects of 20 μmol/L juglone combined with 0, 1.0 or 2.0 μmol/L MG132 (a proteasome inhibitor) on c-Myc expression. The effects of 20 μmol/L juglone on the proliferation and apoptosis of HeLa cells with RNA interference-mediated knockdown of c-Myc were evaluated with MTT assay and flow cytometry.
RESULTS:Treatment with juglone significantly lowered c-Myc protein expression in HeLa cells in a concentration-and time-dependent manner (P < 0.05). Juglone obviously shortened the half-life of c-Myc protein, and the addition of MG132 significantly up-regulated the expression level of c-Myc protein (P < 0.05). Juglone treatment also promoted ubiquitination of c-Myc protein in HeLa cells. Compared with the cells transfected with a negative control construct, the cells transfected with si-c-Myc showed significantly decreased proliferation inhibition and a lowered cell rate with early apoptosis after juglone treatment (P < 0.05).
CONCLUSION:Juglone inhibits proliferation and promotes apoptosis of HeLa cells by affecting the ubiquitination of c-Myc protein.
