Diagnostic efficacy of indirect haemagglutination assay for detection of Schistosoma japonicum infections among boatmen and fishermen in the Dongting Lake region.
10.16250/j.32.1374.2022110
- Author:
Zhong Qiu MEI
1
;
Ting ZENG
1
;
Xin Ting CAI
2
;
Ben Jiao HU
2
;
Sheng Ming LI
2
;
Jie ZHOU
2
;
Tie Wu JIA
1
Author Information
1. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), National Health Commission Key Laboratory of Parasite and Vector Biology, WHO Collaborating Centre for Tropical Diseases, National Center for International Research on Tropical Diseases, Shanghai 200025, China.
2. Hunan Institute of Schistosomiasis Control, China.
- Publication Type:Journal Article
- Keywords:
Boatman and fisherman;
Diagnostic efficacy;
Dongting Lake region;
Indirect haemagglutination assay;
Schistosomiasis
- MeSH:
Animals;
China/epidemiology*;
Hemagglutination;
Humans;
Lakes;
Male;
Middle Aged;
Prevalence;
Schistosoma japonicum;
Schistosomiasis/epidemiology*;
Schistosomiasis japonica/prevention & control*;
Seroepidemiologic Studies
- From:
Chinese Journal of Schistosomiasis Control
2022;34(3):300-306
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To evaluate the diagnostic efficacy of indirect haemagglutination assay (IHA) for detection of Schistosoma japonicum infections among boatmen and fishermen in Dongting Lake region, so as to provide insights into improving the schistosomiasis surveillance program among boatmen and fishermen.
METHODS:The boatmen and fishermen were detected for S. japonicum infections using IHA and Kato-Katz technique or miracidium hatching test nylon gauze simultaneously at schistosomiasis testing sites in the anchor sites for boatmen and fishermen in the Dongting Lake region during the period from 2014 to 2016, and using IHA for serological screening followed by parasitological testing of seropositives during the period from 2017 to 2019. The sensitivity and specificity of IHA were evaluated for detection of S. japonicum infections among boatmen and fishermen, with the 2014-2016 parasitological testing results as a gold standard. In addition, the seroprevalence of S. japonicum infections was compared among boatmen and fishermen with different characteristics and among years.
RESULTS:A total of 306 schistosomiasis testing sites were assigned for boatmen and fishermen, and a total of 143 360 person-time boatmen and fishermen were tested for S. japonicum infections in the Dongting Lake region from 2014 to 2019. The sensitivity and specificity of IHA were 69.9%, 97.3% and 96.1% (χ2 = 74.6, P < 0.05), and 70.9%, 74.5% and 71.9% for detection of S. japonicum infections from 2014 to 2016 (χ2 = 29.4, P < 0.05), respectively. The seroprevalence of S. japonicum infections reduced from 30.3% in 2014 to 1.8% in 2019 among boatmen and fishermen, appearing an overall tendency towards a decline (Z = 1 552.4, P < 0.05). In addition, male, individuals at ages of 45 to 60 years, full-time boatmen and fishermen were more likely to be seropositive for S. japonicum infections (all P values < 0.05).
CONCLUSIONS:The seroprevalence of S. japonicum infections appeared a tendency towards a decline among boatmen and fishermen in the Dongting Lake region year by year from 2014 to 2019. IHA presented a high efficacy for screening of S. japonicum infections among boatmen and fishermen in the Dongting Lake region.
