The role of TSG-6 in inhibiting the secretion of IL-1β by bone-marrow-derived macrophages
10. 20001/j. issn. 2095⁃2619. 20224005
- Author:
zeng zeming
;
fan ruihong
;
nong qiyin
- Publication Type:Journal Article
- Keywords:
Silicon dioxide；Bone-marrow-derived macrophages；Tumor necrosis factor- α -stimulated protein 6；Interleukin 1β；NLRP3；Caspase-1
- From:
China Occupational Medicine
2022;49(2):148-152
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of tumor necrosis factor alpha stimulated gene/inducible protein 6（TSG-6）on
free silica（SiO2
）-induced secretion of pro-inflammatory cytokine interleukin（IL）-1β by bone-marrow-derived macrophages
（BMDMs）. Methods i）BMDMs isolated from bone marrow were divided into eight groups：the control group was untreated；
lipopolysaccharide （LPS） group was stimulated by LPS with a concentration of 50 µg/L；The TSG-6 control group was
stimulated by 100 µg/L of recombinant mouse TSG-6. SiO2 model group was pre-stimulated with LPS for four hours，and then
stimulated with SiO2 suspension at a final concentration of 250 mg/L；Different dose of TSG-6 groups were firstly treated with
above concentrations of LPS and SiO2 suspension，then 10，20，50 and 100 µg/L of recombinant mouse TSG-6 were added
respectively；After 16 hours of culture，the cells were collected and the level of IL-1β in BMDMs supernatant was detected by
enzyme-linked immunosorbent assay（ELISA）to screen optimal concentration of TSG-6. ii）The cells of the control group，LPS
group，SiO2 model group，TSG-6 optimal concentration group and TSG-6 control group were collected. The expression of IL-1β
and components of its related pathways in BMDMs was detected by Western blot，including IL-1β，pro-IL-1β，caspase-1，pro
dcaspase-1，asc type amino acid transport and NOD-like receptor protein 3（NLRP3）. Results i）Compared with the control
group，the expression of IL-1β in SiO2 model group was increased significantly（P<0.01）. Compared with SiO2 model group，the
expression of IL-1β in 20，50，100 µg/L dose of TSG-6 groups were decreased significantly（all P<0.01），and the optimal
concentration of TSG-6 was found to be 100 µg/L. ii）Compared with the control group and LPS group，the relative expression
levels of IL-1β，caspase-1 and NLRP3 in SiO2 model group were increased significantly （all P<0.05）. Compared with
SiO2 model group，the expression levels of IL-1β、caspase-1 and NLRP3 were decreased in 100 µg/L TSG-6 group（all P<0.05）.
Conclusion TSG-6 could inhibit BMDMs to secret pro-inflammatory cytokine IL-1β by down-regulating the expression of
NLRP3 and caspase-1.
