Establishment and Evaluation of Ulcerative Colitis Animal Model in Combination of Disease and Syndrome
10.13422/j.cnki.syfjx.20221592
- VernacularTitle:溃疡性结肠炎病证结合动物模型的制备与评价
- Author:
Yuanyuan YIN
1
;
Donghua BIN
2
;
Ying LIU
1
;
Weiyin ZOU
1
;
Lian YU
1
Author Information
1. Hunan University of Chinese Medicine， Changsha 410208， China
2. The First Affiliated Hospital of Hunan University of Chinese Medicine， Changsha 410007， China
- Publication Type:Journal Article
- Keywords:
ulcerative colitis （UC）;
animal model;
combination of disease and syndrome;
model establishment;
model evaluation
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(15):207-215
- CountryChina
- Language:Chinese
-
Abstract:
Ulcerative colitis （UC） is one of the main manifestations of inflammatory bowel disease. Because of its lingering and refractory nature， it has become a major public health challenge worldwide. In the treatment of UC， traditional Chinese medicine （TCM） effectively relieves clinical syndromes， shortens the treatment period， reduces the frequency of recurrence， improves the quality of life， and reduces the occurrence of complications. To study the specific mechanism of TCM in the treatment of UC and screen out suitable drugs under the guidance of syndrome differentiation， the suitable UC animal model in the combination of disease and syndrome is used as an important method. This paper summarized and compared the UC animal model in the combination of disease and syndrome from five aspects， including selection of model animal species， sexual selection， preparation methods of UC animal model in the combination of disease and syndrome， indicators of model evaluation， and the main mechanism of TCM intervention in UC animal model in the combination of disease and syndrome. This paper aimed to provide references for the establishment of the optimal UC animal model in the combination of disease and syndrome. Research shows that UC syndrome mainly studied at present includes damp-heat syndrome， spleen deficiency syndrome， spleen-kidney Yang deficiency syndrome， spleen deficiency and dampness accumulation syndrome， liver depression and spleen deficiency syndrome， and cold-heat mixed syndrome.In the modeling method， the etiology simulation method is mainly used to first copy the syndrome type before the chemical agents or immune preparations were used to induce the disease model，and rats were often selected as the research objects，and the replication cycle was 7 to 28 days.The selected chemical reagents were mainly 5% dextran sulfate sodium（DSS） free drink, 2,4,6-trinitrobenzenesulfonic acid（TNBS） 100 mg·kg-1 and 50% ethanol 0.25 mL mixed reagent enema.This model replication method can take into account both UC pathogenesis characteristics of pathology of western medicine and TCM， syndrome type of traditional Chinese medicine and western medicine for interpretation pathological changes and TCM treatment of UC associated mechanism is of great significance， and help to help toestablish the optimal condition in combination with UC animal models for reference， for further research on prevention and treatment of UC specific mechanism of action of TCM model basis.
