Mechanism of Chaihu Qinggantang in Intervening NLRP3/IL-1β Pathway to Treat Granulomatous Lobular Mastitis in Rat Model
10.13422/j.cnki.syfjx.20221005
- VernacularTitle:柴胡清肝汤干预NLRP3/IL-1β通路治疗肉芽肿性小叶性乳腺炎模型大鼠的作用机制
- Author:
Yao ZHOU
1
;
Lifang LIU
2
;
Jialu LIU
3
;
Jie GONG
1
;
Shulei LIU
1
;
Dan ZHAO
1
;
Jie LING
1
;
Hongqiao FAN
2
Author Information
1. Hunan University of Chinese Medicine，Changsha 410007，China
2. The First Affiliated Hospital of Hunan University of Chinese Medicine，Changsha 410007，China
3. The Affiliated Hospital of Jiangxi University of Chinese Medicine，Nanchang 330000，China
- Publication Type:Journal Article
- Keywords:
granulomatous lobular mastitis;
animal model of granulomatous lobular mastitis;
Chaihu Qinggantang;
Qingxiao method;
NOD-like receptor thermal protein domain associated protein 3 （NLRP3） inflammasome;
NLRP3/interleukin-1β （IL-1β） signaling pathway
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(15):1-7
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the mechanism of Chaihu Qinggantang （CHQGT） in the treatment of granulomatous lobular mastitis （GLM） in the rat model. MethodSixty female rats were divided into a normal group， a model group， a prednisolone group （0.001 8 g·kg-1）， and three CHQGT low-dose， medium-dose， and high-dose groups （4.5， 8.9， 17.8 g·kg-1）. The tissue homogenates mixed with GLM lesion tissue and Fritner's reagent were used for modeling. After modeling， the treatment groups were given corresponding treatment factors， and the normal group and the model group were given the equal volume of normal saline. The changes in mammary gland of rats were observed after 14 d. Hematoxylin-eosin （HE） staining was used to observe the histopathological changes in breast samples. The mRNA expressions of NOD-like receptor thermal protein domain associated protein 3 （NLRP3） inflammasome， Caspase-1， and interleukin-1β （IL-1β） were detected by real-time quantitative fluorescence polymerase chain reaction （Real-time PCR）. The protein expressions of NLRP3， Caspase-1， IL-1β， and IL-18 were detected by Western bolt. ResultAs compared with the normal group， the breasts of rats in the model group were obviously swelling， and mammary gland inflammation index was significantly increased （P<0.01）. Pathological changes included the formation of granuloma centered on the lobule of mammary gland with a large number of inflammatory cells such as lymphocytes and plasma cells. The mRNA expressions of NLRP3， Caspase-1， and IL-1β， and the protein expressions of NLRP3， Caspase-1， IL-1β， and IL18 in the model group were significantly increased （P<0.01）. Compared with the model group， the treatment groups improved breast swelling， and the CHQGT medium and high-dose groups and the prednisolone group reduced inflammation index to some extent after treatment （P<0.05， P<0.01）. The inflammation degree of mammary gland was significantly improved， and inflammatory cells such as macrophages， lymphocytes， and plasma cells were reduced to varying degrees in pathological aspects. The mRNA expressions of NLRP3， Caspase-1， and IL-1β， and the protein expressions of NLRP3， Caspase-1， IL-1β， and IL-18 in the CHQGT high-dose group and the prednisolone group were significantly down-regulated （P<0.05， P<0.01）. ConclusionCHQGT inhibits inflammation and treats GLM in rats. The mechanism is possibly related to the inhibition of NLRP3/IL-1β signaling pathway， which provides a new target for the prevention and treatment of GLM by Qingxiao method.
