Analysis of Chemical Constituents in Ethanol Extract of Cyclocarya paliurus Dried Leaves by UHPLC-Q-TOF/MS
10.13422/j.cnki.syfjx.20212051
- VernacularTitle:青钱柳醇提物中化学成分的UHPLC-Q-TOF/MS分析
- Author:
Yan XIAO
1
;
Boji MA
1
;
Bingtao LI
1
;
Li JIANG
1
;
Rengeng SHU
1
;
Qiyun ZHANG
1
;
Guoliang XU
1
Author Information
1. Research Center for Differentiation and Development of Traditional Chinese Medicine （TCM） Basic Theory，Jiangxi Provincial Key Laboratory of TCM Etiopathogenisis，Key Laboratory of Pharmacology of TCM in Jiangxi，School of Pharmacy，Jiangxi University of Chinese Medicine，Nanchang 330004，China
- Publication Type:Journal Article
- Keywords:
ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry （UHPLC-Q-TOF/MS）;
Cyclocarya paliurus dried leaves;
chemical constituents;
flavonoids;
triterpenoids;
ethanol extract;
organic acids
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(16):196-203
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveChemical components in ethanol extract of Cyclocarya paliurus dried leaves were analyzed by ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry （UHPLC-Q-TOF/MS）. MethodAn Agilent Poroshell 120 EC-C18 column （3.0 mm×100 mm， 2.7 μm） was used with 0.1% formic acid aqueous solution （A）-acetonitrile （B） as the mobile phase for gradient elution （0-26 min，2%-18%B； 26-60 min， 18%-72%B； 60-70 min， 72%-100%B； 70-71 min， 100%-2%B； 71-72 min， 2%B）， and the flow rate of 0.4 mL·min-1 and injection volume of 3 μL. The electrospray ionization （ESI） was used in positive and negative modes， and detection range was m/z 50-1 100. The collected data were processed by Agilent MassHunter workstation. According to the retention time and MS information of each compound， combined with existing literature and MS database information， the compounds were identified and analyzed for the fragmentation rule. ResultA total of 52， 55 components were identified in the positive and negative ion modes， respectively. Among them， 14 flavonoids， 3 triterpenoids， 15 organic acids and 20 other compounds were identified under positive ion mode， while 18 flavonoids， 9 triterpenoids， 18 organic acids and 10 other compounds were identified under the negative ion mode. By summarizing the positive and negative ion modes and removing the common compounds， 87 compounds were identified， including 22 flavonoids， 27 organic acids， 11 triterpenoids and 27 other compounds. ConclusionUHPLC-Q-TOF/MS can be used to quickly analyze the chemical constituents in C. paliurus dried leaves. 1-Kestose and 18β-glycyrrhetinic acid and other components related to hypoglycemic activity of this herb are identified for the first time， which can provide reference for clarifying the pharmacodynamic substance basis of C. paliurus dried leaves.
