Investigation of Intestinal Absorption Characteristics of Multi-index Components of Danggui Buxuetang Based on Drug Absorption Simulating System
10.13422/j.cnki.syfjx.20220449
- VernacularTitle:基于药物吸收仿生系统的经典名方当归补血汤多指标成分肠吸收特征考察
- Author:
Jia ZHANG
1
;
Yuan GAO
1
;
Xiangjie ZHOU
1
;
Wufeng GAO
1
;
Yue ZHOU
1
;
Lixia MA
1
;
Xinya ZHUANG
1
;
Jie DONG
1
;
Wei GU
1
;
Guojun YAN
1
Author Information
1. School of Pharmacy，Jiangsu Provincial Engineering Research Center of Traditional Chinese Medicine External Medication Development and Application，Jiangsu Provincial Engineering Research Center of Classical Formula，Nanjing University of Chinese Medicine，Nanjing 210023，China
- Publication Type:Journal Article
- Keywords:
drug absorption simulating system （DASS）;
Danggui Buxuetang;
intestinal absorption;
high performance liquid chromatography （HPLC） fingerprint;
cumulative absorption rate;
model fitting;
famous classical formulas
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(16):188-195
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the intestinal absorption characteristics of multi-index components in Danggui Buxuetang with drug absorption simulating system （DASS） established by everted intestinal sac model. MethodThe intestinal absorption solution at different time points after administration of Danggui Buxuetang was collected and detected by high performance liquid chromatography （HPLC）， acetonitrile （A）-0.2% glacial acetic acid solution （B） was used as the mobile phase for gradient elution （0-16 min， 15%-23%A； 16-20 min， 23%-28%A； 20-25 min， 28%-30%A； 25-30 min， 30%A； 30-35 min， 30%-65%A； 35-45 min， 65%-95%A）， the detection wavelength was 302 nm. HPLC fingerprint of intestinal absorption solution was established and the common peak was calibrated， and the relative cumulative absorption rate of each index component was calculated. The relative cumulative absorption curves of components were fitted with various mathematical models by DDSolver 1.0 to explore the absorption law of different components. ResultThe absorption process of C2 （calycosin-7-glucoside） and C6 in Danggui Buxuetang was in line with zero-order equation， C9 was best fitted by Weibull equation， and the remaining 7 components were in line with Makoid-Banakar equation. C1 with C2， C3， C5， C7 and C10， C2 with C5 and C7， C3 with C4， C5， C7 and C10， C4 with C6 and C10， C5 with C7， C6 with C10， C7 with C10， C8 with C9 were absorbed simultaneously during the absorption process. With the prolongation of time， the overall cumulative absorption rate of Danggui Buxuetang increased. At 120 min， the overall cumulative absorption rate of Danggui Buxuetang exceeded 38%， and reached 49.14% at 180 min. ConclusionTen ingredients in Danggui Buxuetang are absorbed in the jejunum， but absorption law of various components is different， which shows that the intestinal absorption of compound preparations of traditional Chinese medicine （TCM） has multiple characteristics. Intestinal absorption study of TCM compound preparations with chemical composition as the index can reveal some of its absorption law， but it is not complete.
