Effect of Organophosphate-Solubilizing Bacteria on Photosynthesis， Physiology， and Biochemistry of Paris polyphylla var. yunnanensis
10.13422/j.cnki.syfjx.20220919
- VernacularTitle:接种解有机磷细菌对滇重楼光合和生理生化的影响
- Author:
Zhuowei LI
1
;
Liangzhong LUO
1
;
Jiaqi LANG
1
;
Mingyan YE
1
;
Fuqiang YIN
1
;
Jingjing ZHAO
1
;
Nong ZHOU
1
Author Information
1. The Chongqing Engineering Laboratory for Green Cultivation and Deep Processing of the Three Gorges Reservoir Area's Medicinal Herbs，College of Food and Biological Engineering Chongqing Three Gorges University，Chongqing 404120，China
- Publication Type:Journal Article
- Keywords:
Paris polyphylla var. yunnanensis;
organophosphate-solubilizing bacteria;
photosynthesis;
physiological and biochemical properties;
compound bacteria
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(16):165-171
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the effect of organophosphate-solubilizing bacteria and compound bacteria on the photosynthesis and physiological and biochemical characteristics of leaves of Paris polyphylla var. yunnanensis， and to provide a reference for selecting suitable bacterial fertilizers in artificial cultivation of this medicinal species. MethodPot experiment was carried out indoor and the following groups were designed： control （CK）， inoculation with Bacillus mycoides （S1）， inoculation with B. wiedmannii （S2）， inoculation with B. proteolyticus （S3）， inoculation with B. mycoides and B. wiedmannii （S4）， inoculation with B. mycoides and B. proteolyticus （S5）， inoculation with B. wiedmannii and B. proteolyticus （S6）， and inoculation with B. mycoides， B. wiedmannii and B. proteolyticus （S7）. Then， the growth and development， photosynthesis， and various physiological and biochemical indexes of the leaves of this species were observed. ResultCompared with CK， the treatment groups showed decrease in content of malondialdehyde in the leaves （P<0.05）， particularly S7 （content was only about 1/3 that of the CK）. The leaf area， photosynthetic parameters， photosynthetic pigment content， soluble sugar content， soluble protein content， and activity of superoxide dismutase （SOD）， peroxidase （POD）， and catalase （CAT） in leaves of the treatment groups were all improved. Among them， the leaf area， soluble sugar content， and soluble protein content were the highest in S7， which were 2.8， 2.1， and 2.2 times that of the CK， respectively. SOD activity peaked in S6 （2.9 times higher than that in the CK） and the highest activity of POD and CAT was detected in S5 （1.5 times and 2.1 times， respectively higher than that in the CK）. ConclusionInoculation with different organophosphate-solubilizing bacteria or compound bacteria can promote the growth and development of P. polyphylla var. yunnanensis and improve its resistance to stresses. The combination of B. mycoides and B. proteolyticus and the combination of the three achieved the have the best effect. This study provides a reference for the selection of bacterial fertilizers for artificial cultivation of P. polyphylla var. yunnanensis.
