Specific PCR Identification of Artemisia absinthium， A New Foreign Medicinal Resource of Artemisia
10.13422/j.cnki.syfjx.20221112
- VernacularTitle:蒿属外来药用资源中亚苦蒿的特异性PCR鉴别
- Author:
Zhihao LIU
1
;
Ziyuan CHEN
2
;
Xiaolin LI
2
;
Chao JIANG
2
;
Yan JIN
2
;
Yuyang ZHAO
2
;
Yuan YUAN
1
Author Information
1. School of Pharmacy， Anhui University of Chinese Medicine， Hefei 230012， China
2. State Key Laboratory Breeding Base of Dao-Di Herbs， National Resource Center for Chinese Materia Medica， China Academy of Chinese Medical Sciences， Beijing 100700， China
- Publication Type:Journal Article
- Keywords:
Artemisia;
Artemisia absinthium;
polymerase chain reaction;
foreign medicinal resources
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(17):127-132
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo establish a specific polymerase chain reaction （PCR） method for the identification of Artemisia absinthium to allow accurate and convenient identification of A. absinthium and its related species. MethodThe chloroplast genome sequences of A. absinthium and its related species were searched from Chloroplast Genome Information Resource （CGIR）， and the specific single nucleotide polymorphism （SNP） sites of A. absinthium were screened out. A pair of specific identification primers （zykh1-F and zykh1-R） of A. absinthium was designed. The original plant samples of A. absinthium and its related species were collected. The specific PCR method was established and optimized， and the tolerance and feasibility of this method were investigated and verified. The method was used to identify A. absinthium samples purchased from Xinjiang medicinal materials market. ResultA 210 bp bright band was obtained from A. absinthium after PCR amplification and gel electrophoresis under the following conditions： specific primers zykh1-F and zykh1-R， annealing temperature of 54 ℃， and the number of cycles of 33. No such band was observed from its relative species， such as A. argyi， A. annua， A. leucophylla， and A. lavandulaefolia. ConclusionThe specific PCR identification method of established in this study can accurately identify A. absinthium and its common related species with high specificity. The method can save time and cost and allows a convenient and fast species identification for the introduction and utilization of A. absinthium resources.
