Effect of Mahonia bealei Leaf Extract on Inflammation in Depression of Rats via NF-κB/NLRP3 Signaling Pathway
10.13422/j.cnki.syfjx.202201005
- VernacularTitle:基于NF-κB/NLRP3通路探究十大功劳叶提取物对抑郁症大鼠炎症的影响及机制
- Author:
Junhui HE
1
;
Fucui ZHU
2
;
Dongmei LI
1
;
Jie WEI
1
;
Jianhua FAN
1
;
Guining WEI
1
Author Information
1. Guangxi Institute of Chinese Medicine and Pharmaceutical Science，Nanning 530022，China
2. Guangxi Key Laboratory of Quality Standard of Traditional Chinese Medicine，Nanning 530022，China
- Publication Type:Journal Article
- Keywords:
Mahonia bealei leaves;
depression;
inflammation;
NOD-like receptor protein 3 （NLRP3）;
nuclear transcription factor-κB （NF-κB）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(17):67-74
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect of Mahonia bealei leaf extract on depression of rats and the underlying mechanism. MethodThe chemical constituents of the extract were analyzed by HPLC-MS/MS. Forced swimming test and tail suspension test were carried out to estimate the antidepressant effect. The mice were randomly assigned into the following groups： blank group， positive control group （fluoxetine， 10 mg·kg-1）， and Mahonia bealei leaf extract groups （10， 2.5 g·kg-1）. The gavage lasted for 12 days and the immobility time of the mice in the tests was recorded 1 h after the last administration. Furthermore， to explore the underlying mechanism of the antidepressant effect， we established the rat depression model by intraperitoneal injection with reserpine （0.5 mg·kg-1）. Rats were grouped as follows： blank group， model group， positive control group （fluoxetine， 1.8 mg·kg-1）， and Mahonia bealei leaf extract groups （10， 2.5 g·kg-1）. The gavage， once a day， lasted for 10 consecutive days. The depression of rats was detected by behavioral tests 1 h after the last administration. The levels of interleukin-6 （IL-6） and tumor necrosis factor-α （TNF-α） in serum of rats were detected by enzyme-linked immunosorbent assay （ELISA）. The protein expression of IL-6 and interleukin-1β （IL-1β） in brain tissue was detected by immunohistochemical staining. The protein levels of nuclear transcription factor-κB （NF-κB） and NOD-like receptor protein 3 （NLRP3） in hippocampus of rats were detected by Western blot. ResultSeven chemical constituents， mainly alkaloids， were identified from the extract. Compared with the blank group， Mahonia bealei leaf extract shortened the immobility time of mice in tail suspension and forced swimming tests. Compared with the blank group， the modeling of rat depression increased the blepharoptosis incidence and retention time in circles （P<0.05， P<0.01）， elevated the levels of IL-6 and TNF-α in serum （P<0.05）， and up-regulated the protein levels of IL-6， IL-1β， NF-κB， and NLRP3 in brain tissues （P<0.01）. Compared with the model group， high dose of Mahonia bealei leaf extract shortened the retention time in circles （P<0.05）， lowered the levels of IL-6 and TNF-α in serum （P<0.05， P<0.01）， and down-regulated the protein levels of IL-6， IL-1β， NF-κB， and NLRP3 （P<0.01） in brain tissues. ConclusionMahonia bealei leaf extract had significant antidepressant effect and alleviated the inflammatory response in reserpine-induced rat model of depression， the mechanism of which may be related to the inhibition of NF-κB/NLRP3 signaling pathway.
