Exploration on Determination and Quantity Transfer of Index Components in Benchmark Samples of Erdongtang
10.13422/j.cnki.syfjx.20220447
- VernacularTitle:经典名方二冬汤基准样品的指标成分含量测定及量值传递规律探索
- Author:
Xiao-xia XUE
1
;
Ru-na JIN
2
;
Xue-yuan WANG
2
;
Shou-gang SHI
3
;
Zheng-jun HUANG
3
;
Yun-tao DAI
1
;
Shi-lin CHEN
2
Author Information
1. Shanxi University of Chinese Medicine，Jinzhong 030619，China
2. Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijng 100700，China
3. Sunflower Pharmaceutical Group （Xiangyang） Longzhong Co. Ltd.，Xiangyang 441003，China
- Publication Type:Journal Article
- Keywords:
classical famous formulas;
Erdongtang;
benchmark samples;
high performance liquid chromatography （HPLC）;
quantity transfer law;
determination;
transfer rate
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(11):1-7
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo establish the determination for index components in benchmark samples of Erdongtang， and clarify the content and transfer rate rages of index components in 15 batches of benchmark samples， and to explore the quantity transfer of index components of decoction pieces to benchmark samples. MethodFifteen batches of benchmark samples were prepared， the contents of mangiferin， baicalin and glycyrrhizic acid were determined by high performance liquid chromatography （HPLC）-diode array detector （DAD）， the mobile phase was acetonitrile （A）-0.1% formic acid aqueous solution （B） for gradient elution （0-10 min， 10%-17%A； 10-25 min， 17%-19%A； 25-28 min， 19%-25%A； 28-45 min， 25%-33%A； 45-46 min， 33%-45%A； 46-60 min， 45%-55%A）， detection wavelength was set at 254 nm. Contents of timosaponin BⅡ and the sum of protoneodioscin and protodioscin were determined by HPLC-evaporative light scattering detector （ELSD）， the mobile phase was acetonitrile （A）-water （B） for gradient elution （0-20 min， 24%A； 20-25 min， 24%-27%A； 25-33 min， 27%-28%A； 33-36 min， 28%-90%A； 36-41 min， 90%-24%A）. ResultThe methodological verification of the established method was good， which could be used for determination of five index components in benchmark samples. The content ranges of mangiferin， baicalin， glycyrrhizic acid， timosaponin BⅡ， and the sum of protoneodioscin and protodioscin in 15 batches of benchmark samples of Erdongtang were 0.14%-0.23%， 2.40%-3.37%， 0.07%-0.44%， 0.43%-0.95%， and 0.15%-0.47%， the transfer rate ranges of them were 33.90%-52.15%， 84.46%-105.61%， 22.59%-93.86%， 38.07%-61.43%， and 53.28%-96.11%， respectively. ConclusionThe consistencies of transfer rate of mangiferin， baicalin， timosaponin BⅡ and the sum of protoneodioscin and protodioscin （except glycyrrhizic acid） between decoction pieces and benchmark samples of Erdongtang are good， indicates that the transfer rates of 4 index components are stable during the preparation process of benchmark samples， which can provide data support for research and development of the compound preparation of this formula.
