Improvement on Quality Standard of Belladonnae Herba
10.13422/j.cnki.syfjx.20211747
- VernacularTitle:颠茄草的质量标准提升
- Author:
Yong LIU
1
;
Ya-lin LIU
1
;
Xue JIANG
1
;
Jin-miao WANG
1
;
Qing-mei FENG
1
;
Hui-min GAO
2
;
Zhen-guo LI
3
;
Er-ping XU
1
;
Li-ping DAI
1
;
Zhi-min WANG
1
Author Information
1. Henan Key Laboratory for Modern Research on Zhongjing's Herbal Formulae，Engineering Research Center for Comprehensive Development and Utilization of Authentic Medicinal Materials from Henan， Henan University of Chinese Medicine，Zhengzhou 450046，China
2. Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China
3. Henan Provincial Institute of Food and Drug Control，Zhengzhou 450012，China
- Publication Type:Journal Article
- Keywords:
Belladonnae Herba;
quality standard;
thin layer chromatography （TLC）;
high performance liquid chromatography （HPLC）;
hyoscyamine sulfate;
scopolamine hydrobromide;
ethanol-soluble extract
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(2):175-181
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo improve the current standard of Belladonnae Herba in the 2020 edition of Chinese Pharmacopoeia. MethodTaking hyoscyamine sulfate， atropine sulfate and scopoletin as reference substances， and ethyl acetate-methanol-concentrated ammonia（17∶4∶2）as developing solvent， thin layer chromatography （TLC） was applied in the qualitative identification of Belladonnae Herba. The moisture， total ash and ethanol-soluble extract of Belladonnae Herba were determined based on the general principles in the 2020 edition of Chinese Pharmacopoeia （volume Ⅳ）. The contents of hyoscyamine sulfate and scopolamine hydrobromide were analyzed by high performance liquid chromatography （HPLC） with mobile phase of acetonitrile-54 mmol·L-1 phosphate buffer solution （14∶86）， flow rate of 1.0 mL·min-1 and detection wavelength at 210 nm. ResultThe spots in the TLC were clear with good separation and specificity. Hyoscyamine sulfate and scopolamine hydrobromide showed a good linearity with peak area in the range of 0.024 7-0.789 6 g·L-1 （r=0.999 9） and 0.003 9-0.124 0 g·L-1 （r=0.999 9）， the average recoveries of these two ingredients were 100.29% （RSD 1.6%） and 99.04% （RSD 1.4%）， respectively. The limits for moisture， total ash in Belladonnae Herba should be less than 13.0% and the limit for the ethanol-soluble extract should be more than 10.0%. Due to the low content and wide variation of scopolamine hydrobromide， the content of hyoscyamine sulfate should not be less than 0.098%. ConclusionThe established method is simple， specific and reproducible， which can be used to improve the quality control standard of Belladonnae Herba.
