Effect of Danggui Niantongtang on Autophagy and Apoptosis of Rheumatoid Arthritis Fibroblast-like Synoviocyte
10.13422/j.cnki.syfjx.20220236
- VernacularTitle:当归拈痛汤对类风湿关节炎成纤维样滑膜细胞自噬与凋亡的影响
- Author:
Qi-jin LU
1
;
Jia-yu LI
2
;
Yi-si CAI
1
;
Hong-xin LIN
1
;
Dan-dan FAN
3
;
Li-gang JIE
3
;
Li-xia YUAN
1
Author Information
1. School of Traditional Chinese Medicine，Southern Medical University，Guangzhou 510515，China
2. School of Laboratory Medicine and Biotechnology，Southern Medical University，Guangzhou 510515， China
3. Zhujiang Hospital of Southern Medical University， Guangzhou 510260， China
- Publication Type:Journal Article
- Keywords:
Danggui Niantongtang;
rheumatoid arthritis;
fibroblast-like synoviocytes;
autophagy;
apoptosis
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(2):19-26
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the effect of Danggui Niantongtang （DGNTT） on cell apoptosis and autophagy in rheumatoid arthritis （RA） fibroblast-like synoviocytes （FLS）. MethodRA-FLS were isolated and cultured from the synovial tissue of RA patients. The cells were treated with 10% blank serum （blank control group）， 10% sera containing low， medium and high doses of DGNTT. Wound healing assa and cell invasion test were applied to observe the effect of RA-FLS invasion technique. The apoptosis and autophagy level of RA-FLS cells was detected by Hoechst33342 method and monodansylcadaverine （MDC） staining. The mRNA and protein expression levels of B cell lymphoma-2 （Bcl-2）， Bcl-2 associated X protein （Bax）， microtubule-associated protein 1 light chain 3 （LC3）， autophagy key molecular yeast Atg6 homolog 1 （Beclin1） were detected by Real-time fluorescence quantitative polymerase chain reaction（Real-time PCR）and Western blot. ResultCompared with the blank control group，each dose of serum could slow down the wound healing and significantly Reduce the number of RA-FLS cells invading the lower chamber（P<0.01），the mRNA and protein expression levels of Bcl-2，LC3，Beclin1 were significantly decreased（P<0.01）， and Bax were significantly increased（P<0.01）. Hoechst33342 results showed that low， medium and high doses DGNTT could promote RA-FLS cell apoptosis. After MDC staining，autophagosome in low， medium and high doses DGNTT decreased significantly（P<0.01）. ConclusionDanggui Niantongtang can effectively inhibit the proliferation of fibroblast-like synoviocytes. Its mechanism may be related to promote apoptosis and inhibit autophagy of fibroblast-like synoviocytes.
