Underlying Mechanisms of Bile Acids in Progression of Hypertension Based on Metabolomics and Network Analysis
10.13422/j.cnki.syfjx.20220311
- VernacularTitle:基于代谢组学和网络分析探讨胆汁酸在高血压进展中的潜在作用机制
- Author:
Yu-chen QI
1
;
Lin-zhen ZOU
1
;
Xue-yan HAN
1
;
Hai-qiang JIANG
1
;
Yun-lun LI
2
Author Information
1. Shandong University of Traditional Chinese Medicine（TCM），Jinan 250355，China
2. Affiliated Hospital of Shandong University of TCM，Jinan 250014，China
- Publication Type:Journal Article
- Keywords:
prehypertension;
bile acid;
vascular inflammation;
metabolomics;
network analysis
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(5):178-186
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the underlying mechanism of bile acids and metabolites as well as the key metabolic pathways and important endogenous targets in prehypertension. MethodThe metabolic mechanism of prehypertension was explored with non-targeted metabolomics combined with network analysis. The serum metabolomics of patients with prehypertension was analyzed by ultra-high performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry. The relevant biological functions and signal targets were predicted and generated by network analysis. Finally，the predicted targets of this important pathway were verified by in vitro experiments，and the relevant information was verified by enzyme-linked immunosorbent assay （ELISA） and Western blot. ResultAs revealed by non-targeted metabolomics，there were 64 potential biomarkers and 13 metabolic pathways in the normal group，the prehypertension group， and the hypertension group. The results of network analysis and biological verification showed that the occurrence of prehypertension was related to vascular inflammation caused by the abnormal metabolism of bile acids and aromatic amino acids. Bile acid metabolism plays an important role in the occurrence and development of prehypertension by regulating the vascular inflammatory response. Amino acid N-acyltransferase，myeloperoxidase， and bile acid downstream receptor TGR5 are critical in the changes of the metabolic network. ConclusionIn prehypertension，bile acids are presumedly involved in regulating vascular inflammation， resulting in damage to blood vessels in prehypertension.
