Effect of Ethyl Acetate Extract of Acanthopanacis Senticosi Radix et Rhizoma seu Caulis on Intestinal Flora in ApoE-/- Mice with Atherosclerosis
10.13422/j.cnki.syfjx.20220152
- VernacularTitle:刺五加乙酸乙酯部位对ApoE-/-动脉粥样硬化小鼠肠道菌群的影响
- Author:
Ai-ling JIA
1
;
Yu-hang ZHANG
2
;
Yuan-yuan DIAO
2
;
Yu-wen SHI
2
;
Meng-cheng JIANG
2
;
Jun-ge LI
2
;
Ye QIU
2
;
Zhi-dong QIU
2
;
Chao-mei FU
1
Author Information
1. School of Pharmacy，Chengdu University of Traditional Chinese Medicine（TCM），Chengdu 611137，China
2. School of Pharmaceutical Sciences，Key Laboratory of TCM Active Ingredient，Ministry of Education，Changchun University of Chinese Medicine，Changchun 130117，China
- Publication Type:Journal Article
- Keywords:
Acanthopanacis Senticosi Radix et Rhizoma seu Caulis;
ethyl acetate extract;
intestinal flora;
apolipoprotein E gene knockout （ApoE-/-） mice;
atherosclerosis;
inflammatory factor;
antioxidant activity;
vascular endothelial factor
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(5):108-115
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the effect of ethyl acetate extract of Acanthopanacis Senticosi Radix et Rhizoma seu Caulis on high-fat diet-induced apolipoprotein E gene knockout （ApoE-/-） mice， and explore its mechanism of treating atherosclerosis by regulating intestinal flora. MethodThirty-two 8-week-old male ApoE-/- mice were randomly divided into model group， rosuvastatin group （10 mg·kg-1）， high-， low-dose groups of ethyl acetate extract of Acanthopanacis Senticosi Radix et Rhizoma seu Caulis （75， 25 mg·kg-1）， with 8 mice in each group. Eight C57BL/6 mice were used as blank group. After 8 weeks of continuous administration， blood was taken to determine the blood lipid level. Enzyme-linked immunosorbent assay （ELISA） was used to detect the contents of related indexes in serum of mice. Hematoxylin-eosin （HE） staining was used to observe the formation of aortic plaque in mice. Cecal contents were collected and 16S rRNA amplicon sequencing was used to detect intestinal flora. ResultCompared with the blank group， the plaque area of the model group was significantly increased with inflammatory infiltration， the contents of triglyceride （TG）， total cholesterol （TC）， low-density lipoprotein cholesterol （LDL-C）， inflammatory factors and inducible nitric oxide synthase （iNOS） were increased， while the content of high-density lipoprotein cholesterol （HDL-C） was decreased. Compared with the model group， rosuvastatin group and high- and low-dose groups of ethyl acetate extract of Acanthopanacis Senticosi Radix et Rhizoma seu Caulis could improve the deposition of aortic plaque， reduce the contents of TG， TC， LDL-C， inflammatory factors and iNOS， and increase the content of HDL-C. Compared with the blank group， the relative abundances of Firmicutes and Proteobacteria in the model group increased， while the relative abundance of Bacteroidetes decreased. Alpha and Beta diversity analysis showed that samples of each group could be significantly isolated， and the total number and abundance of intestinal flora species in the model group were low. Compared with the model group， ethyl acetate extract of Acanthopanacis Senticosi Radix et Rhizoma seu Caulis could increase the relative abundance of beneficial bacteria and decrease the relative abundance of pathogenic bacteria. ConclusionEthyl acetate extract of Acanthopanacis Senticosi Radix et Rhizoma seu Caulis was mainly composed of flavonoids， which can treat atherosclerosis by regulating the intestinal flora and improve the pathological changes in the aorta of ApoE-/- mice induced by high-fat diet. The mechanism may be related to its ability to reduce the level of inflammatory factors， improve antioxidant capacity and repair the disorder of intestinal flora structure.
