Effects of Exogenous Fusarium oxysporum and Trichoderma viride on Soil Fungal Community of Panax ginseng
10.13422/j.cnki.syfjx.20220317
- VernacularTitle:外源添加镰刀菌和木霉菌对农田栽参土壤真菌群落的影响
- Author:
Jing FANG
1
;
Li-li WENG
1
;
Chun-ping XIAO
1
;
Di LI
1
;
Xue-qing YANG
1
Author Information
1. School of Pharmaceutical Sciences， Changchun University of Chinese Medicine， Changchun 130117，China
- Publication Type:Journal Article
- Keywords:
Panax ginseng;
cultivated Panax ginseng in the farmland;
rhizosphere soil;
high-throughput sequencing;
Biolog-ECO
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(6):114-123
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effects of exogenous Fusarium oxysporum and Trichoderma viride on the diversity of soil fungal community and carbon metabolic function of cultivated Panax ginseng. MethodIllumina HiSeq 2500 high-throughput sequencing combined with Biolog-ECO was used to analyze the species diversity and functional diversity of soil fungal communities in P. ginseng soil under different exogenous treatments. ResultThe results of high-throughput sequencing showed that the number and species of microorganisms in the soil were significantly changed after exogenous microorganisms were added. The soil fungi with relative abundance greater than 1% included Mortierella sp.，Fusarium sp.，Humicola sp.，and Simplicillium sp. Mortierella sp. in each treatment group significantly increased. Humicola sp. and Simplicillium sp. could be induced to increase by exogenous addition of F. oxysporum，while T. viride at a high concentration could significantly inhibit the growth of F. oxysporum. As revealed by Biolog and principal component analysis （PCA），the average well color development （AWCD） in the high-dose T. viride group （MG） was significantly higher than that in the control group （QS）and the low-dose F. oxysporum group（LD）. The utilization abilities for amino acids，carboxylic acids，polymers， and amines were enhanced in the MG group，but the microbial metabolic activity was reduced in the high-dose F. oxysporum group （LG）. There was no significant increase in the utilization of phenolic acids by soil microorganisms in both groups. ConclusionExogenous addition of F. oxysporum can lead to the growth and reproduction of other pathogenic fungi. Exogenous addition of T. viride can enhance the soil fungal community structure and metabolic diversity，inhibit the proliferation of F. oxysporum，and improve the soil microbial environment of cultivated P. ginseng.
