Jiangzhi Tongluo Soft Capsules Regulate SIRT1/FoxO3 Pathway to Affect Cell Apoptosis in Rats with Membranous Nephropathy
10.13422/j.cnki.syfjx.20220739
- VernacularTitle:降脂通络软胶囊调控SIRT1/FoxO3通路对膜性肾病大鼠细胞凋亡的影响
- Author:
Fang ZHAO
1
;
Fei GAO
1
;
Shao-hui LI
1
;
Guan-wen ZHANG
1
;
Miao TAN
2
;
Feng-wen YANG
3
;
Mei-fang REN
3
;
Jin-chuan TAN
3
Author Information
1. Hebei University of Chinese Medicine,Shijiazhuang 050200 ,China
2. The Fourth Hospital of Hebei Medicine University,Shijiazhuang 050000,China
3. Hebei Provincial Hospital of Chinese Medicine, Shijiazhuang 050011,China
- Publication Type:Journal Article
- Keywords:
Jiangzhi Tongluo soft capsule;
silent mating-type information regulation 2 homolog 1 （SIRT1）;
forkhead transcription factor O3（FoxO3）;
membranous nephropathy;
podocyte apoptosis;
curcumin extract
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(7):113-120
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the effect of Jiangzhi Tongluo soft capsule on the protein levels of silent mating-type information regulation 2 homolog 1 （SIRT1） and forkhead transcription factor FoxO3 and podocyte apoptosis in the renal tissue of rats with membranous nephropathy and to reveal the underlying molecular mechanisms for the treatment of MN. MethodSixty male SD rats were randomly assigned into 6 groups with 10 rats each. The six groups included a normal group， a model group， benazepril hydrochloride group， and Jiangzhi Tongluo soft capsule groups of low， medium and high doses （25， 50， 100 mg·kg-1， respectively）. The model rats were established by injection with cationized bovine serum albumin into the tail vein. After modeling， the rats were administrated with corresponding agents by gavage for 4 weeks. At the end of the 4th week， an electron microscope was used to observe the pathological changes in the kidney. Western blot was employed to detect the protein levels of SIRT1 and FoxO3 protein in rat kidney， and immunohistochemistry to detect the expression of B lymphocytoma-2 （Bcl-2）， Bcl-2-associated X protein （Bax）， Bcl-2-associated death promoter （Bad）， and podocyte split diaphragm proteins nephrin and podocin. ResultCompared with normal group， the expression of pro-apoptotic factors Bax， Bad， and FoxO3 in the kidney was up-regulated （P<0.05）， while that of anti-apoptotic factors Bcl-2， SIRT1， nephrin， and podocin was down-regulated （P<0.05） after modeling. Compared with the model group， the treatments down-regulated the expression of Bax， Bad， and FoxO3 （P<0.05） and up-regulated that of Bcl-2， SIRT1， nephrin， and podocin （P<0.05）. ConclusionJiangzhi Tongluo soft capsule may regulate the SIRT1/FoxO3 pathway to reduce podocyte apoptosis and maintain podocyte structure stability， thereby exerting the renal protection effect.
