Immunomodulatory and Antioxidant Activity of Gouqi Chewable Tablets
10.13422/j.cnki.syfjx.20220801
- VernacularTitle:枸杞咀嚼片免疫调节及抗氧化活性分析
- Author:
Zheng-xiao SUN
1
;
Shun-li XIAO
1
;
Lu LIU
1
;
Jing XU
1
;
Xiu-fen WANG
2
;
Qiong-ling ZHANG
1
;
Jia-chen SHEN
3
;
Shi-lan DING
1
;
Yun YOU
1
Author Information
1. Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China
2. Department of Biological and Pharmaceutical Technology，Ningxia Polytechnic，Yinchuan 750021，China
3. Guang'anmen Hospital，China Academy of Chinese Medical Sciences， Beijing 100053，China
- Publication Type:Journal Article
- Keywords:
Gouqi chewable tablets;
immunomodulatory;
antioxidant;
phagocytosis
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(8):46-53
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the regulatory effect of Gouqi chewable tablets on innate and adaptive immunity in normal mice and its antioxidant activity in vitro and in vivo. MethodThe effects of low-， medium-， and high-dose groups （0.25， 0.5， 1.5 g·kg-1） on the immune function of normal mice were observed by carbon clearance test， immune organ index test， serum hemolysin test， ConA-induced splenic lymphocyte proliferation test， and natural killer cell （NK cell） activity test. The effects of Gouqi chewable tablets on the antioxidant capacity in vivo were determined by detecting the content of superoxide dismutase （SOD）， glutathione peroxidase （GSH-Px）， and malondialdehyde （MDA） in mice serum. The in vitro antioxidant activity of Gouqi chewable tablets was detected by 2，2'-azino-bis（3-ethylbenzothiazoline-6-sulfonic acid） diammonium salt （ABTS）， 2，2-diphenyl-1-picrylhydrazyl （DPPH）， and hydroxyl radical scavenging tests. ResultCompared with the blank control group， the low-， medium-， and high-dose groups of Gouqi chewable tablets improved the viability of NK cells， the proliferation of splenic lymphocytes， and the level of serum hemolysin antibody in mice （P<0.05）. The high-dose group increased the thymus index， spleen index， and phagocytic function of macrophages （P<0.05， P<0.01）. As compared with the blank control group， the activity of GSH-Px in mice serum in the medium-dose group was increased （P<0.05）， and the content of MDA in mice serum in the high-dose group was decreased （P<0.05）. In in vitro antioxidant tests， the median inhibitory concentration （IC50） values of Gouqi chewable tablets were 1.64±0.20， 2.04±0.03， and 10.27±0.03 g·L-1 by the DPPH， ABTS， and OH- free radical method， respectively. Those results indicated that Gouqi chewable tablets have good antioxidant effects in vitro. ConclusionGouqi chewable tablets can enhance the immune function of mice with good antioxidant effects.
