Effect and Mechanism of Prunellae Spica on Metabolic Syndrome in Rats
10.13422/j.cnki.syfjx.20220416
- VernacularTitle:夏枯草对果糖诱导的大鼠代谢综合征的影响及其代谢机制
- Author:
Yang-qian-nan TANG
1
;
Zi-yu CHEN
1
;
Li LI
1
;
Dun YANG
1
;
Xiao-ye WANG
2
;
Ming-xia XIE
1
Author Information
1. Hunan University of Chinese Medicine， Changsha 410208，China
2. Hunan Brain Hospital （The Second People's Hospital of Hunan Province），Changsha 410007，China
- Publication Type:Journal Article
- Keywords:
Prunellae Spica;
fructose-induced;
metabolic syndrome;
metabolomics;
gas chromatography-mass spectrometry （GC-MS）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(9):125-132
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveMetabolic syndrome is the inherent phenotype of many diseases， which seriously endangers the cardio-cerebrovascular system. Prunellae Spica can regulate lipid metabolism disorder in high-fat mice and inhibit the metabolic disorder of liver injury. This study analyzed the effect of Prunellae Spica on metabolic syndrome and its mechanism， and it is of great significance to find potential safe drugs from natural products. MethodIn this study， the metabolic syndrome model was induced by fructose. The metabolomics method based on gas chromatography-mass spectrometry （GC-MS） was used to explore the effect and mechanism of Prunellae Spica on rats with metabolic syndrome. ResultPharmacological results showed that Prunellae Spica significantly reduced the body weight， blood lipid level and lipid peroxidation level and inhibited the release of tumor necrosis factor-α （TNF-α） in rats with metabolic syndrome. Thus， Prunellae Spica protected the liver and maintained its normal functions. Multivariate statistical analysis revealed that metabolites in the serum of rats with metabolic syndrome changed significantly， which was improved after Prunellae Spica treatment. Compared with the metabolites in normal group， 11 differential metabolic markers were found in rats with metabolic syndrome. Compared with model group， Prunellae Spica group had 8 significantly different metabolic markers， among which phosphate， pyruvic acid and succinic acid were common markers. Pathway analysis indicated that the regulatory effect of Prunellae Spica was mainly related to citrate cycle， glycolysis and gluconeogenesis， serine/threonine and glycine metabolic pathways. ConclusionPrunellae Spica can be used as a potential natural source for the treatment of metabolic syndrome. It can regulate the metabolic disorder in metabolic syndrome via energy and amino acid metabolism.
