Proliferation of Neural Precursor Cells in Dentate Gyrus of Hippocampus and Effect of Yangxue Qingnao Granule in Vascular Dementia Rats
10.3969/j.issn.1006-9771.2015.09.009
- VernacularTitle:血管性痴呆大鼠海马齿状回神经前体细胞增殖的动态变化及养血清脑颗粒的影响
- Author:
Jing LI
;
Ying LIU
;
Bin LIU
;
Yongwei LUO
;
Ying FAN
;
Yuanyuan MA
;
Wenjing MAO
;
Shiying LI
- Publication Type:Journal Article
- Keywords:
vascular dementia, neural precursor cells, proliferation, dentate gyrus, hippocampus, Yangxue Qingnao Granule
- From:
Chinese Journal of Rehabilitation Theory and Practice
2015;21(9):1025-1030
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe dynamic variation of neural precursor cells in dentate gyrus of hippocampus and effect of Yangxue Qingnao Granule on it in vascular dementia rats. Methods 72 Sprague-Dawley rats were randomly divided into sham group (n=24), vascular dementia group (model group, n=24) and Yangxue Qingnao Granule group (treatment group, n=24). The vascular dementia model was established with modified Pulsineli's four-vessel occlusion. The expression of Nestin was detected with Western blotting, the expression of 5-bromodeoxyuridine (BrdU) and BrdU/Nestin were detected with immunofluorescence in dentate gyrus of hippocampus 1, 2, 4 and 8 weeks after modeling. Results The expression of Nestin, BrdU and BrdU/Nestin increased in the model and treatment groups with time, peaked at 4 weeks after modeling, and it was more than that of the sham group on all the time points (P<0.01). However, it was more in the treatment group than in the model group on all the time points (P<0.01). Conclusion Yangxue Qingnao Granule promotes the proliferation of neural precursor cells in dentate gyrus of hippocampus in vascular dementia rats.
