Mechanism of Xianglian Huazhuo Prescription Against Chronic Atrophic Gastritis Based on Network Pharmacology and Experimental Verification
10.13422/j.cnki.syfjx.20220614
- VernacularTitle:基于网络药理学和实验验证探讨香连化浊方对慢性萎缩性胃炎的作用机制
- Author:
Jie WANG
1
;
Yunxiao GAO
1
;
Hongyu MA
2
;
Xuemei JIA
1
;
Yuxi GUO
1
;
Pengli DU
3
;
Danyang ZHAO
3
;
Tong ZHANG
4
;
Bolin LI
3
;
Qian YANG
3
Author Information
1. Hebei University of Chinese Medicine，Shijiazhuang 050091，China
2. Hebei General Hospital，Shijiazhuang 050051，China
3. Hebei Province Hospital of Traditional Chinese Medicine，Shijiazhuang 050011，China
4. The Fourth Hospital of Hebei Medical University，Shijiazhuang 050011，China
- Publication Type:Journal Article
- Keywords:
Xianglian Huazhuo prescription;
chronic atrophic gastritis;
network pharmacology;
mechanism
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(18):161-168
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the mechanism of Xianglian Huazhuo prescription in the treatment of chronic atrophic gastritis （CAG） based on network pharmacology and animal experiments，so as to provide scientific basis for clinical application. MethodThe possible targets and pathways of Xianglian Huazhuo prescription in the treatment of CAG were obtained based on the prediction of network pharmacology. The CAG rat model was induced by sodium salicylate，N-methyl-N'-nitro-N-nitrosoguanidine （MNNG） and hunger and satiety disorder. Then the CAG rats were treated with Xianglian Huazhuo prescription and morodan for 60 days. After administration，the rats were sacrificed，and the content of interleukin-6 （IL-6），tumor necrosis factor-α （TNF-α），interleukin-1β （IL-1β） and vascular endothelial growth factor （VEGF） in serum was determined by enzyme linked immunosorbent assay（ELISA）. In addition， the protein expression of Bad and Bcl-2 in gastric mucosa was detected by immunohistochemistry （IHC）. ResultA total of 241 active components of Xianglian Huazhuo prescription and 53 core targets were obtained. Xianglian Huazhuo prescription affected multiple biological processes，such as cell proliferation and apoptosis，inflammatory reaction，regulation of DNA metabolism，and cell response to redox，as well as phosphatidylinositol 3-kinase/protein kinase B （PI3K/Akt），TNF，mitogen-activated protein kinase （MAPK），cancer and cancer-related signaling pathways. The animal model verification showed that Xianglian Huazhuo prescription lowered the levels of IL-6，TNF-α，IL-1β and VEGF in serum of CAG rats，and reduced the protein expression of Bad and Bcl-2 in gastric tissue. ConclusionXianglian Huazhuo prescription could regulate PI3K/Akt signal pathway and improve gastric mucosal injury in CAG by participating in biological processes such as cell proliferation，apoptosis and inflammation.
