Identification and Expression Analysis of PP2C Gene Family Members in Cannabis sativa
10.13422/j.cnki.syfjx.20221111
- VernacularTitle:工业大麻PP2C基因家族成员鉴定及表达分析
- Author:
Xiaoxue CAI
1
;
Sifan WANG
2
;
Yaolei MI
2
;
Huihua WAN
2
;
Xue CAO
2
;
Wei SUN
2
;
Chang SU
3
;
Shilin CHEN
2
;
Yanqin XU
1
;
Weiqiang CHEN
2
Author Information
1. School of Pharmacy，Jiangxi University of Chinese Medicine，Nanchang 330004，China
2. Institute of Chinese Materia Medica，Chinese Academy of Chinese Medical Science，Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine，Beijing 100700，China
3. Shenzhen Institute for Drug Control，Shenzhen 518057，China
- Publication Type:Journal Article
- Keywords:
hemp;
type 2C protein phosphatases （PP2C）gene family;
gene identification;
functional prediction;
expression analysis
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(19):162-172
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveThe type 2C protein phosphatases （PP2C） are involved in numerous plant signal transduction pathways. They mainly participate in plant stress response and regulate second metabolites biosynthesis via negatively regulating MAPK signaling pathway. Herein，we were to identify and analyze PP2C （CsPP2C） gene family from hemp genome，in hope of providing comprehensive insights for studying CsPP2C function during the development of hemp. MethodMolecular Evolutionary Genetics Analysis （MAGA）-X was used to construct phylogenetic tree. Expert Protein Analysis System （ExPASy），WoLF PSORT，Multiple EM for Motif Elicitation （MEME），Batch Conserved Domain Search （Batch-CD-Search），PlantCare，and TBtools were used，respectively，to predict CsPP2C physicochemical properties，subcellular localization，conserved motifs，protein structure，cis-element in promoter and collinearity with Arabidopsis PP2C. Cannabis sativa transcriptome and Real-time polymerase chain reaction（Real-time PCR） were used to analyze and verify gene expressions，respectively. ResultFifty-two CsPP2C with conserved domains were identified from the entire genome of hemp，encoding proteins ranging from 244 to 1 089 aa in length and with molecular weights ranging from 26.76 to 122.53 kDa. Those genes were mainly distributed in the nucleus，cytoplasm and chloroplast. The 47 CsPP2C were divided into 10 subfamilies，and the remaining 5 were not clustered. Seven pairs of homologous genes between hemp and Arabidopsis thaliana were identified according to collinear analysis. The light-responsive elements and abscisic acid elements are most abundant in the prediction. The gene expression heat map showed varied expression pattern of CsPP2C in different tissues. Real-time PCR results of three CsPP2C were consistent with transcriptome data. Moreover，alternative splicing analysis showed that some CsPP2C had alternative-splicing genes during evolution. ConclusionWe predicted and analyzed CsPP2C gene family in genomic scale and showed that CsPP2C are involved in many biological processes，whereby provides foundation for CsPP2C functional study.
