Scutellariae Barbatae Herba Extract Promotes Cycle Arrest of Nasopharyngeal Carcinoma Cells by Inhibiting STAT3/SKP2 Signaling Pathway
10.13422/j.cnki.syfjx.20221227
- VernacularTitle:半枝莲提取物通过抑制STAT3/SKP2信号通路促进鼻咽癌细胞周期阻滞
- Author:
Hong DING
1
;
Zilu WU
1
;
Jitang CAI
2
;
Jingbo LI
2
Author Information
1. Second Clinical Medical College， Henan University of Chinese Medicine， Zhengzhou 450002， China
2. Henan Province Hospital of Traditional Chinese Medicine， Zhengzhou 450002， China
- Publication Type:Journal Article
- Keywords:
Scutellariae Barbatae Herba;
nasopharyngeal carcinoma;
cell cycle arrest;
signal transducer and activator of transcription 3 （STAT3）;
S-phase kinase associated protein 2 （SKP2）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(19):81-88
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the effect of Scutellariae Barbatae Herba extract on on the cycle arrest of nasopharyngeal carcinoma cells and the possible mechanism by adding different concentration of Scutellariae Barbatae Herba extract （0.25， 0.5， 1 g·L-1） in the culture medium， taking CNE1 （nasopharyngeal carcinoma cells） as the research object. MethodAfter the treatment of CNE1 by Scutellariae Barbatae Herba extract， cell counting kit-8 （CCK-8） was used to detect cell proliferation， and Giemsa staining was used to detect the clone formation rate. Flow cytometry was used to detect cell cycle distribution， and reverse transcription-polymerase chain reaction （ RT-PCR） assay and Western blot assay were used to detect the relative expression of messenger ribonucleic acid （mRNA） by small interfering RNA （siRNA） or overexpression. ResultAs compared with the blank group， the proliferation and colony formation rate of CNE1 in the Scutellariae Barbatae Herba extract group significantly decreased （P<0.05， P<0.01） in a dose and time-dependent manner， whereas the percentage of cells in the presynthetic phase （G0/G1） increased （P<0.05， P<0.01）. The expression level of S-phase kinase associated protein 2 （SKP2） in the Scutellariae Barbatae Herba extract group significantly decreased （P<0.01） as compared with the blank group. As compared with the Scutellariae Barbatae Herba extract group， the protein levels of p21 and p27 significantly decreased in the overexpressed SKP2+ Scutellariae Barbatae Herba extract group （P<0.01）. As compared with the blank group， the signal activation and the phosphorylation level of signal transducer and activator of transcription 3 （STAT3） of CNE1 in the S. barbata extract group significantly decreased （P<0.05， P<0.01）. ConclusionScutellariae Barbatae Herba extract effectively inhibits the proliferation of CNE1， and the mechanism may be related to its action on the STAT3/SKP2 pathway.
