Protective Effect of Water Extract of Citri Grandis Exocarpium on Alcohol-induced Acute Liver Injury
10.13422/j.cnki.syfjx.20221190
- VernacularTitle:化橘红水提物对酒精诱导的急性肝损伤保护作用
- Author:
Daoshun WU
1
;
Mengchen WANG
1
;
Xuelian ZHANG
1
;
Yifei GUO
1
;
Zhengqi DONG
1
;
Yanhui WANG
2
;
Yun LUO
1
;
Xiaobo SUN
1
Author Information
1. Institute of Medicinal Plant Development，Chinese Academy of Medical Sciences，Beijing 100193，China
2. Guangzhou Xiangxue Pharmaceutical Co. Ltd.，Guangzhou 510663，China
- Publication Type:Journal Article
- Keywords:
water extract of Citri Grandis Exocarpium;
alcohol-induced acute liver injury;
enzyme expression level;
hepatocyte apoptosis;
hepatic protection
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(19):42-48
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the pharmacodynamic effect of the water extract of Citri Grandis exocarpium （WEC） on mice with alcohol-induced acute liver injury and provide data support for the development of this medicinal for anti-alcoholism and liver protection. MethodThe main components of WEC were determined by high performance liquid chromatography （HPLC）. Sixty Balb/c mice were randomized into 6 groups： control group （equal volume of 0.5% carboxymethyl cellulose sodium solution）， model group （equal volume of 0.5% carboxymethyl cellulose sodium solution）， low-， medium-， and high-dose WEC groups （0.5， 1.0， 2.0 g·kg-1）， and Haiwang Jinzun tablet positive control group （2.0 g·kg-1）. The administration lasted 14 days. One day before the end of the administration， mice were fasted for 12 h with free access to water. The mice， except the control group， were given 56° Chinese liquor （13 mL·kg-1）. After 2 h， blood was taken from eyeballs and the liver was dissected and weighed. Automatic biochemical analyzer was employed to detect the expression of alanine aminotransferase （ALT）， aspartate aminotransferase （AST）， and alcohol dehydrogenase （ADH）. The pathological changes of liver tissues were observed based on hematoxylin-eosin （HE） staining， and apoptosis of hepatocytes based on TUNEL/DAB staining. The expression of proteins related to apoptosis was detected by Western blot. ResultAccording to the HPLC fingerprint， the main components of WEC were rhoifolin and naringin. Compared with the control group， the model group showed increase in liver/body weight ratio （P<0.01） and the expression of ALT and AST （P<0.05， P<0.01）， decrease in the expression of ADH （P<0.05）， blurred structure of hepatic lobules， pathological changes of liver tissue， loose cytoplasm with edema， severe steatosis， rise of the TUNEL-positive rate （P<0.01）， reduction in expression of Bcl-2 （P<0.01）， and increase in Bax and Caspase-3 （P<0.01）. Compared with the model group， medium-dose WEC lowered liver/body weight ratio （P<0.05）. All doses of WEC depressed the activity of ALT and AST （P<0.05， P<0.01）， up-regulated the expression of ADH （P<0.05）， significantly improved the pathological features of alcohol-induced cytoplasmic porosity， edema， and steatosis， down-regulated the TUNEL-positive rate （P<0.05， P<0.01）， enhanced the expression of Bcl-2 （P<0.05）， and decreased Bax and Caspase-3 （P<0.01）. ConclusionWEC regulates the expression of ALT， AST， and ADH and improves hepatic steatosis and hepatocyte apoptosis to fight against acute liver injury.
