Autophagic Cell Death in Glioma Cell Induced by Ceramide through JNK/c-Jun Pathway
10.3969/j.issn.1006-9771.2015.08.007
- VernacularTitle:神经酰胺通过JNK/c-Jun信号通路诱导胶质瘤细胞自噬性死亡①
- Author:
Luyong ZHANG
;
Miao ZHANG
;
Shibu LIU
;
Rui LI
- Publication Type:Journal Article
- Keywords:
glioma cell, ceramide, autophagic cell death, JNK signaling pathway
- From:
Chinese Journal of Rehabilitation Theory and Practice
2015;21(8):905-912
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the autophagy of 87-MG and U251 glioma cells induced by ceramide and explore the possible mechanism. Methods The viability and apoptosis of 87-MG and U251 cells were detected by MTT assay and flow cytometry, respectively. Autophagic- related protein expressions of LC3B /LC3A and Beclin-1 were determined by Western blotting. The activation of JNK/c-Jun signaling pathway induced by ceramide with or without the treatment of JNK specific inhibitor SP600125 was also measured. Results 24 hours after treatment of ceramide, the growth of 87-MG and U251 cells was significantly inhibited time-dependently (P<0.05); and the number of autophagic cells increased dose-dependently (P<0.05). The levels of LC3B/LC3A and Beclin-1 significantly increased after ceramide treatment (P<0.05). JNK signaling pathway was activated in the 87-MG and U251 cells and the phosphorylation of c-Jun also increased after ceramide treatment. This activation of autophagy could be reversed by the pre-treatment of SP600125. Conclusion Ceramide may induce autophagy in 87-MG and U251 glioma cells and the mechanism may be related to the activation of JNK/c-Jun signaling pathway.
