Evaluation of antitrypanosomal properties and apoptotic effects of ochrolifuanine from Dyera costulata (Miq.) Hook.f against Trypanosoma brucei brucei
https://doi.org/10.47665/tb.39.3.003
- Author:
Norhayati, I.
1
;
Nurhayati, Z.A.
2
;
Getha, K.
1
;
Muhd Haffiz, J.
1
;
Adiratna, M.R.
3
Author Information
1. Natural Product Division, Forest Research Institute Malaysia, 52109 Kepong, Selangor, Malaysia
2. Institute of Science Biology (ISB), University Malaya, 50603 Kuala Lumpur, Malaysia
3. Immunology Research Centre, Institute of Medical Research (IMR), 50580, Kuala Lumpur, Malaysia
- Publication Type:Journal Article
- Keywords:
Ochrolifuanine;
Dyera costulata;
apoptotic;
Trypanosoma brucei brucei.
- From:Tropical Biomedicine
2022;39(No.3):321-327
- CountryMalaysia
- Language:English
-
Abstract:
Trypanosoma brucei parasites are flagellated kinetoplastid protozoan which is responsible for Human
African Trypanosomiasis (HAT). Current chemotherapy drugs have a number of side effects and drug
resistance has emerged as a major issue in current treatment. Active bisindole alkaloid compound
ochrolifuanine was previously isolated from the leaves of Dyera costulata. In vitro antitrypanosomal
activity of ochrolifuanine against Trypanosoma brucei brucei strain BS221 showed strong activity with
an IC50 value of 0.05 ± 0.01 µg/ml. We compared the effect of ochrolifuanine and reference compound
staurosporine in T. b. brucei apoptosis. The apoptosis-inducing activity of ochrolifuanine was evaluated
using TUNEL assay and cell cycle analysis. Trypanosoma brucei brucei was shown to undergo apoptotic
cells death as demonstrated by the appearance of several conical hallmarks of apoptosis. Ochrolifuanine
was found to induce apoptosis in parasites in a dose- and time-dependent manner. The cell cycle study
revealed 0.025 and 0.05 µg/ml of ochrolifuanine arrested the growth of T. b. brucei at two different
growth phases (G0/G1 and in S phases). While at concentration 0.10 µg/ml arrested at the G2/M phase.
In conclusion, the results indicate that ochrolifuanine displayed an antitrypanosomal effect on T. b. brucei
by inducing apoptosis cell death and causing the arrest of parasite cells at different growth phases. The
results suggested that ochrolifuanine may be a promising lead compound for the development of new
chemotherapies for African trypanosomiasis.
- Full text:8.2022my1348.pdf
