A new species of black fly, formerly cytoform C of the Simulium angulistylum complex (Diptera: Simuliidae), from a high mountain in northeastern Thailand
https://doi.org/10.47665/tb.39.2.018
- Author:
Pramual, P.
1
;
Jomkumsing, P.
1
;
Thongyan, T.
1
;
Wongpakam, K.
2
;
Takaoka, H.
3
Author Information
1. Department of Biology, Faculty of Science, Mahasarakham University, Kantharawichai District, Mahasarakham 44150, Thailand
2. Walai Rukhavej Botanical Research Institute, Mahasarakham University, Kantharawichai District, Mahasarakham, 44150, Thailand
3. Higher Institution of Centre of Excellence (HICoE), Tropical Infectious Diseases Research and Education Centre (TIDREC), Universiti Malaya, 50603, Kula Lumpur, Malaysia
- Publication Type:Journal Article
- Keywords:
cytoform;
DNA barcode;
species complex;
taxonomy.
- From:Tropical Biomedicine
2022;39(No.2):281-290
- CountryMalaysia
- Language:English
-
Abstract:
Cytoform C, one of three cytoforms in the Simulium angulistylum Takaoka & Davies complex from
a high mountain in northeastern Thailand was morphologically and molecularly investigated. All
known life stages (larva, pupa, adult male and female except egg) were morphologically similar to, but
distinguishable from S. angulistylum s. str. and S. isanense Takaoka, Srisuka & Saeung in the adults by
the relative length of the fore and hind basitarsi and relative length of the tooth to the claw. It is also
morphologically distinct from other species of the S. epistum species-group. Here, it is formally described
as a new species, S. prayooki. Molecular genetic data based on mitochondrial cytochrome c oxidase
subunit I (COI) also supported the morphological similarity between the new species and the two related
known species (S. angulistylum s. str. and S. isanense) as phylogenetic analysis retrieved them all from
a single clade and with a low level of interspecific genetic divergence (1.74%). This might possibly have
resulted from incomplete lineage sorting as they are likely to share a recent common ancestor. Despite
limitation of molecular genetic differentiation, the new species was distinctly different from two other
cytoforms of S. angulistylum complex based on polytene chromosome banding patterns and ecology
of the immature stages. Thus, this study highlights the necessity of using an integrated approach for
fully understanding black fly biodiversity.
- Full text:8.2022my13431.pdf
