Effect of Activating Blood to Resolve Stagnation on Cells Expressing CD34 and Vascular Endothelial Growth Factor in Rats with Acute Myocardial Infarction
10.3969/j.issn.1006-9771.2015.08.005
- VernacularTitle:活血化瘀法对急性心肌梗死大鼠CD34、血管内皮生长因子阳性细胞的影响①
- Author:
Wei SHI
;
Yezi LI
;
Haibin ZHAO
;
Dandan YANG
;
Tianyuan JIANG
;
Dongfang LI
;
Yaoyao ZHAI
- Publication Type:Journal Article
- Keywords:
acute myocardial infarction, marrow stem cells, vascular endothelial growth factor, micro environment, Activating Blood to Resolve Stagnation method
- From:
Chinese Journal of Rehabilitation Theory and Practice
2015;21(8):894-899
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of Activating Blood to Resolve Stagnation on the expression of CD34 and vascular endothelial growth factor (VEGF) in rats with acute myocardial infarction. Methods 32 Sprague-Dawley rats were randomly divided into sham operation group (A, n=8), model group (B, n=8), Xuesaitong Injection + granulocyte colony- stimulating factor (G- CSF) group (C, n=8) and G-CSF group (D, n=8). Corresponding medicine was given to each group 3 hours after modeling, for 6 days. Pathomorphological changes were observed through HE staining, and the expression of CD34, VEGF and Ki-67 were observed through immunohistochemical staining. Results The expressions of CD34, VEGF and Ki-67 were higher in groups B, C and D than in group A (P<0.05), and were higher in group groups C and D than in group B (P<0.05). The expressions of CD34 and VEGF were higher in group C than in group D (P<0.05). However, there was no significant difference in the expression of Ki-67 between 2 groups (P>0.05). Conclusion The expression of CD34 and VEGF increases with Activating Blood to Resolve Stagnation method, which is superior to using G-CSF only. Activating Blood to Resolve Stagnation may play an important role in the treatment of acute myocardial infarction.
