Isolation, identification and electrophysiological activity of BmK M2 from Buthus martensii Karsch venom
10.11665/j.issn.1000-5048.20220413
- VernacularTitle:蝎毒素BmK M2的分离鉴定及其电生理活性研究
- Author:
Ming SANG
1
;
Yonggen CHEN
;
Qian ZHOU
;
Peng CAO
;
Wuguang LU
Author Information
1. 南京中医药大学附属中西医结合医院
- Publication Type:Journal Article
- Keywords:
Buthus martensii Karsch;
BmK M2;
Nav1.7;
whole-cell patch clamp;
electrophysiological activity;
isolation;
identification
- From:
Journal of China Pharmaceutical University
2022;53(4):498-506
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed to isolate and identify novel toxin peptides targeting voltage-gated sodium channels (VGSGs) from the venom of the Buthus martensii Karsch (BmK) scorpion. Using G50-gel filtration, HPLC, peptide fingerprinting and amino acid sequencing, a novel sodium channel modulator, BmK M2, was identified from BMK scorpion. BmK M2 is a relatively abundant long chain polypeptide toxin in BmK scorpion venom with a molecular weight of 7 235.59, consisting of 64 amino acids and 4 pairs of disulfide bonds.Sequence alignment showed that the amino acid sequence of BmK M2 had high sequence and structural similarity to that of the discovered sodium channel toxins of BmK M1, BmK M3 and BmK M9, etc.BmK M2 is a potential new sodium channel modulator.Electrophysiological results revealed that BmK M2 can significantly enhance the activation, delay the steady-state inactivation and closed-state inactivation of Nav1.7, but has no activity on Nav1.8.BmK M2 can be used as a novel peptide probe for the study of the structure and function of Nav1.7 and the development of drugs targeting Nav1.7.
