Identification of chemical constituents in Fufang Shechuangzi Xiji（Lotion）by UPLC-Q-TOF-MS/MS
10.11665/j.issn.1000-5048.20220408
- VernacularTitle:基于UPLC-Q-TOF-MS/MS技术分析复方蛇床子洗剂中的化学成分
- Author:
Xiaohong CHENG
1
;
Li JIANG
;
Xingting DONG
;
Xiulin KANG
;
Lifang LIU
Author Information
1. 中国医学科学院皮肤病医院
- Publication Type:Journal Article
- Keywords:
Fufang Shechuangzi Xiji（Lotion）;
UPLC-Q-TOF-MS/MS;
chemical constituents;
effective substances
- From:
Journal of China Pharmaceutical University
2022;53(4):452-466
- CountryChina
- Language:Chinese
-
Abstract:
Fufang Shechuangzi Xiji (Lotion) is a common hospital preparation made from clinical prescriptions. Its clinical efficacy is accurate, yet its pharmacodynamic material basis has not been clarified.An ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS) technique was developed to identify the chemical constituents of Fufang Shechuangzi Xiji (Lotion).The chemical constituents of Fufang Shechuangzi Xiji (Lotion) were scanned by electrospray ionization (ESI) source in positive and negative ion modes.As a result, a total of 118 compounds were identified and characterized via reference standards and by comparing mass spectrometry data with literature, including 45 alkaloids, 21 coumarins and chromones, 19 flavonoids, 14 saponins, 10 anthraquinones and 9 organic acids.As a result, UPLC-QTOF-MS/MS technology can quickly and sensitively identify the chemical constituents of Fufang Shechuangzi Xiji (Lotion), which provides a useful reference for exploring the pharmacodynamic material basis and further quality control study of the preparation.
