Deep chemical identification of phytoecdysteroids in Achyranthes bidentata Blume by UHPLC coupled with linear ion trap-Orbitrap mass spectrometry and targeted isolation.
10.1016/S1875-5364(22)60185-7
- Author:
Ying-Ying WANG
1
,
2
;
Jia-Yuan LI
3
;
Chang-Liang YAO
3
;
Jian-Qing ZHANG
3
;
Yang YU
4
,
5
;
Shuai YAO
3
;
Min GAO
4
,
5
;
Shi-Fei WU
3
;
Wen-Long WEI
3
;
Qi-Rui BI
3
;
De-An GUO
1
,
6
Author Information
1. School of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing 210023, China
2. Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
3. Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
4. Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China
5. School of Pharmaceutical Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.
6. Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China. Electronic address: daguo@simm.ac.cn.
- Publication Type:Journal Article
- Keywords:
Achyranthes bidentata Blume;
Chemical identification;
Fragmentation behaviors;
Phytoecdysteroids;
Targeted isolation
- MeSH:
Achyranthes/chemistry*;
Chromatography, High Pressure Liquid/methods*;
Gas Chromatography-Mass Spectrometry;
Mass Spectrometry;
Medicine, Chinese Traditional;
Spectrometry, Mass, Electrospray Ionization/methods*
- From:
Chinese Journal of Natural Medicines (English Ed.)
2022;20(7):551-560
- CountryChina
- Language:English
-
Abstract:
Achyranthes bidentata Blume is widely used as a traditional Chinese medicine with the effects of nourishing the liver and kidneys and strengthening muscles and bones. In this work, a rapid and simple strategy was developed for characterizing phytoecdysteroids by ultra-high-performance liquid chromatography coupled with liner ion trap-Orbitrap mass spectrometry using electrospray ionization in the negative mode. As a result, 47 phytoecdysteroids were unambiguously or tentatively characterized. Among them, seven known compounds were identified according to the reference standards along with molecular formula, retention time and fragmentation patterns, while others were mostly potential new compounds. Through targeted isolation, the structures of three new compounds were determined by NMR spectra, which were consistent with LC-MS characterization. The present study provides an efficient method to deeply characterize phytoecdysteroids.
