Anti-pseudo-allergic components in licorice extract inhibit mast cell degranulation and calcium influx.
10.1016/S1875-5364(22)60148-1
- Author:
Lu WANG
1
;
Gui-Zhou HU
2
;
Yin LU
3
;
Shu-Jun JIANG
4
;
Jin QI
5
;
Hua SU
6
Author Information
1. National Center of Colorectal Disease, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing 210022, China. Electronic address: wanglu@njucm.edu.cn.
2. Jiangsu Key Laboratory of TCM Evaluation and Translation Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China.
3. Department of Pharmacy, Jinling Hospital, Nanjing 210002, China.
4. National Center of Colorectal Disease, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing 210022, China.
5. Jiangsu Key Laboratory of TCM Evaluation and Translation Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China. Electronic address: qijin2006@163.com.
6. Department of Pharmacy, Jinling Hospital, Nanjing 210002, China. Electronic address: suhuanjzyy@163.com.
- Publication Type:Journal Article
- Keywords:
Anti-pseudo-allergy;
Glycyrrhiza uralensis;
Glycyrrhizic acid;
Licorice chalcone A;
MAS-related G protein-coupled receptor-X2
- MeSH:
Animals;
Anti-Allergic Agents/therapeutic use*;
Calcium/metabolism*;
Cell Degranulation;
Glycyrrhiza;
HEK293 Cells;
Humans;
Hypersensitivity/drug therapy*;
Mast Cells/metabolism*;
Mice;
Mice, Inbred C57BL;
Molecular Docking Simulation;
Nerve Tissue Proteins/metabolism*;
Rats;
Receptors, G-Protein-Coupled/metabolism*;
Receptors, Neuropeptide/therapeutic use*
- From:
Chinese Journal of Natural Medicines (English Ed.)
2022;20(6):421-431
- CountryChina
- Language:English
-
Abstract:
Pseudo-allergic reactions (PARs) widely occur upon application of drugs or functional foods. Anti-pseudo-allergic ingredients from natural products have attracted much attention. This study aimed to investigate anti-pseudo-allergic compounds in licorice. The anti-pseudo-allergic effect of licorice extract was evaluated in rat basophilic leukemia 2H3 (RBL-2H3) cells. Anti-pseudo-allergic compounds were screened by using RBL-2H3 cell extraction and the effects of target components were verified further in RBL-2H3 cells, mouse peritoneal mast cells (MPMCs) and mice. Molecular docking and human MRGPRX2-expressing HEK293T cells (MRGPRX2-HEK293T cells) extraction were performed to determine the potential ligands of MAS-related G protein-coupled receptor-X2 (MRGPRX2), a pivotal target for PARs. Glycyrrhizic acid (GA) and licorice chalcone A (LA) were screened and shown to inhibit Compound48/80-induced degranulation and calcium influx in RBL-2H3 cells. GA and LA also inhibited degranulation in MPMCs and increase of histamine and TNF-α in mice. LA could bind to MRGPRX2, as determined by molecular docking and MRGPRX2-HEK293T cell extraction. Our study provides a strong rationale for using GA and LA as novel treatment options for PARs. LA is a potential ligand of MRGPRX2.
